Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	169.3 / NA	43	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/26);  total (17/26)

GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382510‑1382601                                              |                                               gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                             >  1:3436282/1‑49 (MQ=255)  cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:1391550/1‑50 (MQ=255)   gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:2280962/50‑1 (MQ=255)    aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:1339505/50‑1 (MQ=255)    aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:3716057/50‑1 (MQ=255)      tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:1402876/50‑1 (MQ=255)      tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       >  1:1656721/1‑50 (MQ=255)         aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:815178/1‑50 (MQ=255)         aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:3914646/50‑1 (MQ=255)             ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:983722/49‑1 (MQ=255)              tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               >  1:1949093/1‑50 (MQ=255)                  tcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            >  1:3847533/1‑49 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                             <  1:3115118/46‑1 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:1698963/48‑1 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          <  1:310188/49‑1 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          <  1:2110240/49‑1 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:1880905/1‑50 (MQ=255)                     aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:1679684/50‑1 (MQ=255)                     aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:3167086/50‑1 (MQ=255)                       cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCa                      <  1:3234939/50‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:540574/50‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:3424171/50‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:4324188/50‑1 (MQ=255)                               cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:184221/49‑1 (MQ=255)                               cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              >  1:3702197/1‑50 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:1319320/1‑50 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:3354827/49‑1 (MQ=255)                                 atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            <  1:1208995/50‑1 (MQ=255)                                 atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            >  1:2780834/1‑50 (MQ=255)                                   atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:856090/1‑50 (MQ=255)                                   atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:3479075/1‑50 (MQ=255)                                    tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:4336372/49‑1 (MQ=255)                                    tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:1560285/1‑50 (MQ=255)                                     ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:201548/1‑49 (MQ=255)                                       acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:4071497/50‑1 (MQ=255)                                       acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:2878422/50‑1 (MQ=255)                                       acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:2375078/50‑1 (MQ=255)                                        cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     >  1:451606/1‑50 (MQ=255)                                        cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:517283/50‑1 (MQ=255)                                        cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     >  1:2945985/1‑50 (MQ=255)                                         gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:3546456/50‑1 (MQ=255)                                          acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:1366747/50‑1 (MQ=255)                                           cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  >  1:142055/1‑50 (MQ=255)                                              |                                               GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382510‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑