Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	234.8 / NA	59	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/27);  total (32/27)

GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477105‑3477196                                              |                                               gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:4149412/49‑1 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:1952969/1‑50 (MQ=255)  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:1687197/1‑49 (MQ=255)  aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                           <  1:1374682/50‑1 (MQ=255)   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:4105517/1‑48 (MQ=255)   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:861338/50‑1 (MQ=255)     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:1047959/1‑49 (MQ=255)      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:3081398/49‑1 (MQ=255)       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:3572976/1‑49 (MQ=255)       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:3061124/1‑49 (MQ=255)       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       >  1:358734/1‑49 (MQ=255)        tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:3310516/1‑50 (MQ=255)         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:3221327/49‑1 (MQ=255)         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    >  1:86673/1‑50 (MQ=255)          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:1821124/49‑1 (MQ=255)          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:4199546/50‑1 (MQ=255)          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:3061191/50‑1 (MQ=255)          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  1:3148431/1‑50 (MQ=255)             agTCTGTTCGATCTCTTGGTGGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:2452814/50‑1 (MQ=255)             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:823702/50‑1 (MQ=255)             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:674314/50‑1 (MQ=255)                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:248041/1‑49 (MQ=255)                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            >  1:3865477/1‑49 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:2438655/47‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:1687323/48‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            <  1:3769492/48‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:2657590/50‑1 (MQ=255)                    tCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:3272181/1‑49 (MQ=255)                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:3562132/1‑50 (MQ=255)                       aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:2926340/1‑49 (MQ=255)                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:1017190/1‑48 (MQ=255)                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:1447292/1‑48 (MQ=255)                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:20234/1‑50 (MQ=255)                          tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   >  1:332162/1‑50 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       <  1:1569909/44‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:2171806/46‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:299846/47‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:3725094/48‑1 (MQ=255)                             tGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:535391/1‑50 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt                >  1:2450732/1‑48 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:2320608/49‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:133782/49‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:2242060/49‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:436285/50‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:964210/50‑1 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:2811505/1‑50 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:2324323/1‑49 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:181030/1‑49 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:3120667/1‑49 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg          <  1:3582227/50‑1 (MQ=255)                                     gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt        <  1:3381148/50‑1 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:2313702/1‑50 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       <  1:4215195/50‑1 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:738021/1‑50 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:902513/1‑50 (MQ=255)                                         ttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt     >  1:2139513/1‑49 (MQ=255)                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:3641799/1‑50 (MQ=255)                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:462133/1‑50 (MQ=255)                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:2137940/1‑50 (MQ=255)                                              |                                               GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477105‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑