Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	155.0 / NA	39	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048                                              |                                              ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            <  1:934239/49‑1 (MQ=255)   aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:3006550/1‑49 (MQ=255)       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:1627612/49‑1 (MQ=255)       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:4302280/49‑1 (MQ=255)        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:4078103/1‑50 (MQ=255)        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  1:1963600/50‑1 (MQ=255)          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:4082545/1‑50 (MQ=255)          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:1717754/1‑50 (MQ=255)           aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:1879325/50‑1 (MQ=255)              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:1708589/1‑49 (MQ=255)              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:607688/1‑49 (MQ=255)              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              <  1:122868/50‑1 (MQ=255)                aCGTTTGATTTTCTTCGCTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:856912/50‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  1:3624527/1‑50 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:42046/1‑50 (MQ=255)                   tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGCTAACGt                         >  1:2985295/1‑50 (MQ=255)                   tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         <  1:3193458/50‑1 (MQ=255)                       aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     <  1:3485930/50‑1 (MQ=255)                           tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  1:1990252/49‑1 (MQ=255)                            cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  1:2328025/49‑1 (MQ=255)                            cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  1:3684642/49‑1 (MQ=255)                              tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:4112717/1‑50 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:3128257/48‑1 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:3359384/1‑50 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             <  1:2354801/49‑1 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:728401/50‑1 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:1317351/50‑1 (MQ=255)                                 cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           >  1:4009402/1‑50 (MQ=255)                                 cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           <  1:2296037/50‑1 (MQ=255)                                  tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2176860/1‑50 (MQ=255)                                     cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2460330/1‑47 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:1221710/1‑50 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:2330266/1‑50 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:531103/1‑50 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:1103765/50‑1 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:3439113/1‑49 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:1098323/49‑1 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:4111284/49‑1 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:2314189/1‑49 (MQ=255)                                              |                                              GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑