Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	295.6 / NA	73	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/43);  total (30/43)

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857                                                |                                               catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:5036686/1‑50 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                             <  1:796983/50‑1 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:702128/1‑49 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:3574783/1‑49 (MQ=255)   tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGAATGTTa                                            <  1:4942071/50‑1 (MQ=39)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:1843766/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           <  1:2814161/49‑1 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  1:4053578/50‑1 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  1:2807771/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:5847568/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:2107908/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:4701889/50‑1 (MQ=255)        tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       <  1:3845162/50‑1 (MQ=255)         aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:4754008/1‑50 (MQ=255)         aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:3110178/1‑50 (MQ=255)         aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:5913019/1‑50 (MQ=255)          ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:464180/50‑1 (MQ=255)          ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:439606/50‑1 (MQ=255)          ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:3973794/50‑1 (MQ=255)           gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:2864417/50‑1 (MQ=255)            cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:2108797/50‑1 (MQ=255)            cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                   <  1:1617523/50‑1 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  1:5736219/50‑1 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 >  1:2861169/1‑50 (MQ=255)                aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:5798462/50‑1 (MQ=255)                 aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:2893879/1‑48 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:212116/1‑47 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:2000692/50‑1 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             <  1:4044736/50‑1 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:4920350/1‑48 (MQ=255)                    aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:1585395/1‑48 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  1:4420136/50‑1 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:979556/1‑50 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  1:3795596/50‑1 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          <  1:2584129/50‑1 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:3920938/50‑1 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                         <  1:788451/50‑1 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:5100560/1‑49 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:473071/1‑49 (MQ=255)                       gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:2257253/1‑48 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:653354/1‑47 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:5572834/49‑1 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:5189692/49‑1 (MQ=255)                         cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:3552094/1‑46 (MQ=255)                         cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:3575935/50‑1 (MQ=255)                          aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  1:3780551/1‑49 (MQ=255)                          aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGat                     <  1:322683/50‑1 (MQ=255)                           gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    >  1:1075413/1‑50 (MQ=255)                            aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  1:2856120/1‑50 (MQ=255)                             tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:681864/50‑1 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 <  1:5871646/49‑1 (MQ=255)                               cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGc                <  1:5475865/50‑1 (MQ=255)                                 ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:5572240/1‑49 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:5520210/1‑48 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:471661/48‑1 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:3689186/49‑1 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:1512631/50‑1 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:1773648/1‑50 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:1191894/1‑50 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:5115108/1‑50 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:541119/50‑1 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         <  1:556781/50‑1 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         <  1:2499347/50‑1 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:478953/1‑49 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:3011346/50‑1 (MQ=255)                                        aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        >  1:2446988/1‑49 (MQ=255)                                        aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:2035707/50‑1 (MQ=255)                                           gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:2256538/1‑47 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:106993/1‑48 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  1:1209346/50‑1 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:3270358/49‑1 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag    <  1:3378669/49‑1 (MQ=255)                                             gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:5879520/50‑1 (MQ=255)                                                |                                               CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270764‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑