Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	336.4 / NA	84	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/46);  total (38/46)

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382508‑1382601                                                |                                               ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:628310/1‑50 (MQ=255) ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:2533798/1‑50 (MQ=255) ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              >  1:204789/1‑50 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                              <  1:462393/48‑1 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                             >  1:5143860/1‑49 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           <  1:1250383/50‑1 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:5131760/1‑50 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                            <  1:924037/49‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:4452228/50‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:2548533/50‑1 (MQ=255)      aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:1541315/1‑49 (MQ=255)      aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:1108116/50‑1 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:4242905/50‑1 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:3283400/1‑50 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:5345770/1‑50 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:4219097/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:3414575/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:4865731/50‑1 (MQ=255)           aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    <  1:4571283/50‑1 (MQ=255)           aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:4742150/1‑50 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:3870088/1‑49 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                   <  1:1853419/50‑1 (MQ=255)             tattatCTCGAGCGCGGCTTCAATATGGACTACAGTCGCAATAACGGt                                    >  1:136044/1‑48 (MQ=255)             tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:5304841/1‑48 (MQ=255)              attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 >  1:5983378/1‑50 (MQ=255)              attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 >  1:485028/1‑50 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                  <  1:4562716/48‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:2688848/49‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                >  1:1611321/1‑50 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                <  1:2951189/50‑1 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                <  1:171569/50‑1 (MQ=255)                  tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                             >  1:2958219/1‑50 (MQ=255)                  tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                             >  1:3719510/1‑50 (MQ=255)                  tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              <  1:3710271/49‑1 (MQ=255)                   ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            >  1:3470306/1‑50 (MQ=255)                     cGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          >  1:2206576/1‑50 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                             <  1:1841464/46‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:4814658/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:4966310/50‑1 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:2276041/50‑1 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:5049764/50‑1 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:2251753/50‑1 (MQ=255)                        gcgcGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       <  1:2675140/50‑1 (MQ=255)                          gcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:4433686/1‑48 (MQ=255)                           cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:4466446/1‑47 (MQ=255)                           cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:2494890/50‑1 (MQ=255)                           cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:1964276/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:782078/50‑1 (MQ=255)                             gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaa                  <  1:1082787/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:4348470/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 >  1:1131529/1‑50 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:5009940/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 >  1:1598687/1‑50 (MQ=255)                                tCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               >  1:3696761/1‑50 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:5831556/48‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              >  1:2365509/1‑50 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:4671712/1‑50 (MQ=255)                                  aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:4923973/1‑50 (MQ=255)                                   atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            >  1:5534987/1‑50 (MQ=255)                                   atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            <  1:2249928/50‑1 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:2128630/1‑50 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:590683/1‑50 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:2235456/1‑50 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          <  1:5978082/49‑1 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:2134551/50‑1 (MQ=255)                                       ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:1543671/1‑49 (MQ=255)                                       ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:4308914/1‑49 (MQ=255)                                       ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:4651546/1‑49 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt        <  1:4070852/49‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:2513213/50‑1 (MQ=255)                                        gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       <  1:3494263/50‑1 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      >  1:2337820/1‑50 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     >  1:5549488/1‑50 (MQ=255)                                          cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     >  1:5849327/1‑50 (MQ=255)                                           gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:1689512/50‑1 (MQ=255)                                           gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    >  1:3055530/1‑50 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:5328317/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:4644152/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:4276570/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:4339775/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:673130/50‑1 (MQ=255)                                             cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:2028118/50‑1 (MQ=255)                                             cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:3455719/50‑1 (MQ=255)                                             cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:2994647/50‑1 (MQ=255)                                                |                                               TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382508‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑