Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	295.7 / NA	74	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/37);  total (37/37)

CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCT  >  NC_000913/1811162‑1811253                                               |                                              cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGc                                            >  1:5438142/1‑50 (MQ=255)   aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGccc                                          <  1:2650145/50‑1 (MQ=255)    aTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCg                                         >  1:5730445/1‑50 (MQ=255)    aTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCg                                         <  1:3881693/50‑1 (MQ=255)     tGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGt                                        >  1:3201348/1‑50 (MQ=255)      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                       >  1:2493330/1‑50 (MQ=255)       aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                      >  1:505442/1‑50 (MQ=255)       aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                      >  1:4981820/1‑50 (MQ=255)       aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                      <  1:4655506/50‑1 (MQ=255)        tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                     <  1:4634018/50‑1 (MQ=255)         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                     >  1:526249/1‑49 (MQ=255)         cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                     >  1:523038/1‑49 (MQ=255)          gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGc                                    <  1:890603/49‑1 (MQ=255)           ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                   >  1:5796669/1‑49 (MQ=255)           ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                   >  1:4391808/1‑49 (MQ=255)            tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  >  1:289476/1‑49 (MQ=255)            tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  >  1:3860261/1‑49 (MQ=255)             aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                 <  1:100833/49‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                <  1:4470595/49‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                <  1:2090127/49‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                               <  1:1063482/50‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                               <  1:1495710/50‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                               <  1:2525456/50‑1 (MQ=255)              tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                               <  1:2362113/50‑1 (MQ=255)               gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              >  1:1495888/1‑50 (MQ=255)               gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              >  1:342890/1‑50 (MQ=255)                ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              <  1:1103338/49‑1 (MQ=255)                ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              >  1:705020/1‑49 (MQ=255)                ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              >  1:4007992/1‑49 (MQ=255)                ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGc                             <  1:5633331/50‑1 (MQ=255)                 cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCa                            <  1:574218/50‑1 (MQ=255)                 cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCa                            >  1:1528979/1‑50 (MQ=255)                 cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCa                            >  1:762965/1‑50 (MQ=255)                  gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                           >  1:3926307/1‑50 (MQ=255)                  gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                           <  1:1603709/50‑1 (MQ=255)                   ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                          >  1:4059292/1‑50 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:1481946/49‑1 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:1969656/49‑1 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                         >  1:3256657/1‑48 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCa                       <  1:5920755/50‑1 (MQ=255)                          tCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                     >  1:1368083/1‑48 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                    <  1:5023454/48‑1 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTa                   <  1:6038300/49‑1 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTa                   <  1:5504013/49‑1 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                  >  1:286314/1‑50 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                  >  1:1656957/1‑50 (MQ=255)                            gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 >  1:216598/1‑50 (MQ=255)                            gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 >  1:4731657/1‑50 (MQ=255)                            gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                  <  1:5265582/49‑1 (MQ=255)                             ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 >  1:2689151/1‑49 (MQ=255)                             ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                <  1:4348699/50‑1 (MQ=255)                             ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                <  1:5202827/50‑1 (MQ=255)                              ctctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                >  1:5739320/1‑49 (MQ=255)                              ctctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa               <  1:2816468/50‑1 (MQ=255)                              ctctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGCTAGGCa               <  1:543217/50‑1 (MQ=255)                                 tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa            >  1:2437516/1‑50 (MQ=255)                                 tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa            >  1:4103935/1‑50 (MQ=255)                                 tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa              <  1:4355699/48‑1 (MQ=255)                                   ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt           <  1:2664112/49‑1 (MQ=255)                                   ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt           >  1:3229723/1‑49 (MQ=255)                                    tCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt           >  1:525665/1‑48 (MQ=255)                                     cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCa         <  1:5958487/49‑1 (MQ=255)                                       gAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt      >  1:4960091/1‑50 (MQ=255)                                        aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAg        <  1:5523821/47‑1 (MQ=255)                                        aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAg        <  1:4384020/47‑1 (MQ=255)                                        aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt      <  1:6017994/49‑1 (MQ=255)                                        aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt      <  1:5043001/49‑1 (MQ=255)                                        aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt       <  1:2177465/48‑1 (MQ=255)                                         gcgcGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt    <  1:5367356/50‑1 (MQ=255)                                         gcgcGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt    <  1:2903458/50‑1 (MQ=255)                                          cgcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTc   >  1:3068601/1‑50 (MQ=255)                                           gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt  >  1:4378835/1‑50 (MQ=255)                                           gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt  >  1:2090853/1‑50 (MQ=255)                                            cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt  >  1:3486584/1‑49 (MQ=255)                                               |                                              CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCT  >  NC_000913/1811162‑1811253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑