Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	276.4 / NA	68	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/44);  total (24/44)

GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214839‑214931                                               |                                               gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                              <  1:2298319/49‑1 (MQ=255) gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:5755295/1‑50 (MQ=255)  tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:1227981/1‑49 (MQ=255)    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          <  1:3387346/50‑1 (MQ=255)    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          >  1:2905042/1‑50 (MQ=255)     tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                         <  1:4816163/50‑1 (MQ=255)      ttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        >  1:4820691/1‑50 (MQ=255)      ttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        >  1:2517838/1‑50 (MQ=255)       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        <  1:3415371/49‑1 (MQ=255)        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       <  1:3477871/49‑1 (MQ=255)         ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                      <  1:60489/49‑1 (MQ=255)         ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:2943563/50‑1 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  1:1490889/50‑1 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    >  1:2174080/1‑50 (MQ=255)           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   <  1:5326241/50‑1 (MQ=255)           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   <  1:2389825/50‑1 (MQ=255)           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   <  1:3437605/50‑1 (MQ=255)           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:5871804/1‑50 (MQ=255)           gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:210484/1‑50 (MQ=255)            aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:4009103/1‑49 (MQ=255)             cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:2344487/1‑48 (MQ=255)              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                <  1:4026567/50‑1 (MQ=255)              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                <  1:1449811/50‑1 (MQ=255)               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  1:3411101/50‑1 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                              <  1:2235085/49‑1 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                              <  1:1834135/49‑1 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             >  1:834959/1‑50 (MQ=255)                   gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           >  1:5568668/1‑50 (MQ=255)                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          <  1:5307741/50‑1 (MQ=255)                      aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        >  1:5934501/1‑50 (MQ=255)                       ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       >  1:4256286/1‑50 (MQ=255)                       ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       >  1:4155697/1‑50 (MQ=255)                       ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        <  1:2217863/49‑1 (MQ=255)                        tCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcg                      >  1:1118516/1‑50 (MQ=255)                         cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     >  1:6021171/1‑50 (MQ=255)                         cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcg                      <  1:4783091/49‑1 (MQ=255)                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     <  1:1985321/49‑1 (MQ=255)                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    <  1:760716/50‑1 (MQ=255)                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    <  1:4394832/50‑1 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc                   <  1:1032959/50‑1 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc                   <  1:5135148/50‑1 (MQ=255)                            gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgca                  <  1:550543/50‑1 (MQ=255)                             aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 <  1:2343265/50‑1 (MQ=255)                             aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  1:4090817/1‑50 (MQ=255)                             aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  1:2775593/1‑50 (MQ=255)                                gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca               <  1:3337820/49‑1 (MQ=255)                                gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt              >  1:2703371/1‑50 (MQ=255)                                gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt              <  1:5152656/50‑1 (MQ=255)                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:5760240/50‑1 (MQ=255)                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:2729518/50‑1 (MQ=255)                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:414313/50‑1 (MQ=255)                                  gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:2830046/1‑50 (MQ=255)                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           <  1:1344700/50‑1 (MQ=255)                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           <  1:4061968/50‑1 (MQ=255)                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  1:3760196/49‑1 (MQ=255)                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:2928054/50‑1 (MQ=255)                                    gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:952485/50‑1 (MQ=255)                                     cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:2447200/1‑50 (MQ=255)                                     cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         <  1:5254884/50‑1 (MQ=255)                                      ttGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:672014/1‑49 (MQ=255)                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  1:1179636/49‑1 (MQ=255)                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        <  1:5251940/48‑1 (MQ=255)                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  1:6025493/1‑48 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  1:2664089/49‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  1:4329983/49‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg     <  1:3928649/50‑1 (MQ=255)                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa    <  1:4689203/50‑1 (MQ=255)                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   <  1:2759417/50‑1 (MQ=255)                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  <  1:144038/50‑1 (MQ=255)                                               |                                               GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214839‑214931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑