BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,794	T→C	intergenic (+23/‑35)	frsA → / → crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,794	0	T	C	100.0%	428.9 / NA	103	intergenic (+23/‑35)	frsA/crl	fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (50/53); total (50/53)

Only 100 of 103 total aligned reads displayed.

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              >  1:308284/1‑50 (MQ=255)

cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:2231118/50‑1 (MQ=255)

cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:465458/50‑1 (MQ=255)

cTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTg                                              <  1:1807651/50‑1 (MQ=255)

 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:5978489/1‑50 (MQ=255)

 tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:1823715/1‑50 (MQ=255)

  ggATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             >  1:74065/1‑49 (MQ=255)

   gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTgg                                             <  1:2856799/48‑1 (MQ=255)

    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaa                                          <  1:2704911/50‑1 (MQ=255)

    aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTa                                           >  1:3743349/1‑49 (MQ=255)

      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:1486771/1‑50 (MQ=255)

      cGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:4301619/1‑50 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  1:4314050/49‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  1:1993825/49‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        <  1:1054880/49‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:511669/50‑1 (MQ=255)

       gAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAAc                                       <  1:4662168/50‑1 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:2521038/1‑48 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:5445289/1‑48 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:5029294/1‑48 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTaaaa                                        >  1:3222136/1‑48 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                      <  1:3983463/50‑1 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                      <  1:1458808/50‑1 (MQ=255)

        aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACa                                      <  1:2026031/50‑1 (MQ=255)

         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     >  1:4146679/1‑50 (MQ=255)

         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     >  1:3964770/1‑50 (MQ=255)

         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     >  1:1107656/1‑50 (MQ=255)

         aaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAg                                     <  1:1178028/50‑1 (MQ=255)

          aaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGt                                    >  1:2016684/1‑50 (MQ=255)

           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   <  1:2241373/50‑1 (MQ=255)

           aaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGtt                                   <  1:348006/50‑1 (MQ=255)

            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:1401905/50‑1 (MQ=255)

            aCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  <  1:347388/50‑1 (MQ=255)

             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTg                                  >  1:4212084/1‑49 (MQ=255)

             cGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGc                                 <  1:699775/50‑1 (MQ=255)

              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                <  1:4753645/50‑1 (MQ=255)

              gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCa                                <  1:2851217/50‑1 (MQ=255)

               cTTGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:4711500/1‑50 (MQ=255)

               cTTGTGTTAAAAATTTGCTAAATTTTGCC‑ATCCGGGAAAAAAGTTGCATc                              >  1:4376155/1‑50 (MQ=255)

                ttGTGTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCAt                               >  1:1719313/1‑49 (MQ=255)

                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:1633902/1‑50 (MQ=255)

                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:1303038/1‑50 (MQ=255)

                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:4097491/1‑50 (MQ=255)

                 tgtgTTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATca                             >  1:2234210/1‑50 (MQ=255)

                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                              <  1:5546122/48‑1 (MQ=255)

                  gtgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATc                              <  1:922457/48‑1 (MQ=255)

                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcaca                           <  1:1843475/50‑1 (MQ=255)

                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            <  1:476968/49‑1 (MQ=255)

                   tgtTAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATcac                            >  1:1836524/1‑49 (MQ=255)

                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:5864233/1‑49 (MQ=255)

                     ttAAAAATTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCacaa                          >  1:2658840/1‑49 (MQ=255)

                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:4055066/1‑50 (MQ=255)

                       aaaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:4061227/1‑50 (MQ=255)

                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:6016309/49‑1 (MQ=255)

                        aaaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       <  1:5347053/49‑1 (MQ=255)

                         aaaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAg                       >  1:3096387/1‑48 (MQ=255)

                          aaTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgag                    <  1:855182/50‑1 (MQ=255)

                           aTTTGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   <  1:1756239/50‑1 (MQ=255)

                            tttGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGgaga                   >  1:360303/1‑49 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATa                 <  1:575111/49‑1 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:2801558/1‑50 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:2395953/1‑50 (MQ=255)

                              tGCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                >  1:25387/1‑50 (MQ=255)

                               gCTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAg                <  1:4665288/49‑1 (MQ=255)

                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:6022561/49‑1 (MQ=255)

                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGc               <  1:2877985/49‑1 (MQ=255)

                                cTAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCa              <  1:2016686/50‑1 (MQ=255)

                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  1:1490891/1‑50 (MQ=255)

                                 tAAATTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCaa             >  1:4503170/1‑50 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:3785138/49‑1 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAAt            <  1:2832540/49‑1 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg           <  1:1536715/50‑1 (MQ=255)

                                   aaTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATg           <  1:1884241/50‑1 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:1584460/1‑50 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          >  1:4629180/1‑50 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  1:4633083/50‑1 (MQ=255)

                                    aTTTTGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGa          <  1:2334400/50‑1 (MQ=255)

                                     ttttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGAc         <  1:5354887/50‑1 (MQ=255)

                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        >  1:1990207/1‑50 (MQ=255)

                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        >  1:584571/1‑50 (MQ=255)

                                      tttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        >  1:261355/1‑50 (MQ=255)

                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:3329739/49‑1 (MQ=255)

                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACg        <  1:2962046/49‑1 (MQ=255)

                                       ttGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:1976304/1‑50 (MQ=255)

                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:4195436/1‑49 (MQ=255)

                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       >  1:1536251/1‑49 (MQ=255)

                                        tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGt       <  1:6033861/49‑1 (MQ=255)

                                         gCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGtt      <  1:4023841/49‑1 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTa     <  1:1989993/49‑1 (MQ=255)

                                          ccAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAc    <  1:1109849/50‑1 (MQ=255)

                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2516821/1‑50 (MQ=255)

                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:2415320/1‑50 (MQ=255)

                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:3624251/50‑1 (MQ=255)

                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   <  1:3563103/50‑1 (MQ=255)

                                           cAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:144283/1‑50 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:3906549/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTAcc   >  1:483866/1‑49 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:5564683/50‑1 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:3790993/50‑1 (MQ=255)

                                            aaTCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCg  <  1:3299785/50‑1 (MQ=255)

CTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCG  >  NC_000913/257747‑257840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`