BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	522.5 / NA	127	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (49/78); total (49/78)

Only 100 of 132 total aligned reads displayed.

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:119589/49‑1 (MQ=255)

cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                              <  1:5584628/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:1067640/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                             <  1:2924261/49‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:4475887/50‑1 (MQ=255)

 gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            <  1:4412508/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:2096768/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                            >  1:1514561/1‑48 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:3300475/50‑1 (MQ=255)

   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                          <  1:5623324/50‑1 (MQ=255)

    tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:1869954/1‑50 (MQ=255)

     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                         >  1:3555910/1‑49 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        >  1:4876023/1‑49 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        >  1:918155/1‑49 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        <  1:4063213/49‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                        >  1:4267633/1‑49 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  1:3165801/50‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  1:3035426/50‑1 (MQ=255)

      cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                       <  1:5432424/50‑1 (MQ=255)

        tGTAGAGTCTTTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     >  1:2296285/1‑50 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                     <  1:470259/49‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:919594/48‑1 (MQ=255)

         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                      <  1:4508492/48‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:4678772/49‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:4426356/49‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                    <  1:1383763/49‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   >  1:4874530/1‑50 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:2556841/50‑1 (MQ=255)

          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                   <  1:1597946/50‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:5563961/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                  <  1:3434747/49‑1 (MQ=255)

            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                 >  1:3424327/1‑50 (MQ=255)

             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                <  1:3414692/50‑1 (MQ=255)

             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                                >  1:3287552/1‑50 (MQ=255)

              gTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATc                               >  1:4867603/1‑50 (MQ=255)

                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:390134/1‑50 (MQ=255)

                cTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:2636274/1‑50 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:4146137/1‑49 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:5472557/1‑49 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:4084048/1‑49 (MQ=255)

                 tGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             >  1:1312963/1‑49 (MQ=255)

                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            >  1:4686954/1‑49 (MQ=255)

                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                            >  1:5405420/1‑49 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                             <  1:3618609/47‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:4613916/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:3267878/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:1416663/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          <  1:5876152/50‑1 (MQ=255)

                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                          >  1:1696275/1‑50 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        >  1:3593876/1‑50 (MQ=255)

                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                         <  1:2155145/49‑1 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:1898276/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:3601768/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:200588/1‑50 (MQ=255)

                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                        <  1:4173256/49‑1 (MQ=255)

                       aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:693068/1‑49 (MQ=255)

                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                       >  1:430038/1‑48 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:382694/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:452192/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:5167525/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                      <  1:5102965/45‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:2364187/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:5483517/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                     <  1:2812320/46‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:1948545/47‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                    <  1:3780287/47‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:2253623/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:4545731/48‑1 (MQ=255)

                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                   <  1:5941877/48‑1 (MQ=255)

                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                 <  1:1504146/48‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:1972651/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:201695/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:2500236/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc               <  1:2121924/49‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:3939283/50‑1 (MQ=255)

                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa              <  1:3747176/50‑1 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             >  1:4267435/1‑50 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:5026733/50‑1 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             >  1:3826423/1‑50 (MQ=255)

                                tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg             <  1:4836862/50‑1 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  1:265692/1‑50 (MQ=255)

                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            >  1:4022928/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:2584285/49‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc            <  1:665818/49‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:4994938/1‑50 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           <  1:5217564/50‑1 (MQ=255)

                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt           >  1:629078/1‑50 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:1511502/50‑1 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         <  1:1464097/50‑1 (MQ=255)

                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa         >  1:2895764/1‑50 (MQ=255)

                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc       >  1:2997295/1‑50 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      <  1:4133935/50‑1 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:2652339/1‑50 (MQ=255)

                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt      >  1:4233435/1‑50 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:839922/49‑1 (MQ=255)

                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt    <  1:4545653/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  1:1722870/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa   <  1:2608202/49‑1 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:5963914/1‑50 (MQ=255)

                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAt  >  1:1275380/1‑50 (MQ=255)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTAT  >  NC_000913/3477104‑3477196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`