Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	277.5 / NA	69	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/34);  total (35/34)

CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048                                               |                                              cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:3215097/1‑50 (MQ=255)   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:1886536/1‑50 (MQ=255)    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:4357818/50‑1 (MQ=255)    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:3491783/1‑49 (MQ=255)    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:4115245/1‑49 (MQ=255)    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:5358675/1‑49 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:5892288/1‑48 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:4999423/50‑1 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:4493564/50‑1 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:1405966/50‑1 (MQ=255)       cAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:4039496/1‑49 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:1613972/50‑1 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:5131353/50‑1 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:1722567/49‑1 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:1422897/49‑1 (MQ=255)         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  1:5499615/50‑1 (MQ=255)         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:5582273/1‑50 (MQ=255)         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  1:5220725/50‑1 (MQ=255)          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:2211804/50‑1 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:524521/50‑1 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:1505687/50‑1 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:3233617/50‑1 (MQ=255)             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:825034/1‑49 (MQ=255)             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1811172/1‑49 (MQ=255)               gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:306073/1‑49 (MQ=255)               gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              <  1:4402759/50‑1 (MQ=255)                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:5096004/1‑49 (MQ=255)                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:715846/50‑1 (MQ=255)                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  1:4064061/1‑50 (MQ=255)                 aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  1:5847155/1‑50 (MQ=255)                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  1:5021121/1‑50 (MQ=255)                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:2072275/50‑1 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          <  1:21461/50‑1 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:6011180/1‑50 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          <  1:1666252/50‑1 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:4741074/1‑50 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         <  1:394944/50‑1 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         <  1:5165654/50‑1 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:3304249/1‑50 (MQ=255)                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGtt                        <  1:3782505/49‑1 (MQ=255)                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:3011381/1‑50 (MQ=255)                      tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:4968885/1‑50 (MQ=255)                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       <  1:4485502/49‑1 (MQ=255)                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       <  1:545533/49‑1 (MQ=255)                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:5031866/1‑50 (MQ=255)                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:4767242/1‑50 (MQ=255)                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:429740/1‑50 (MQ=255)                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:3560267/1‑50 (MQ=255)                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    <  1:5048831/50‑1 (MQ=255)                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    >  1:3815228/1‑50 (MQ=255)                         ttttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTg                    <  1:113016/50‑1 (MQ=255)                          tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:4574839/1‑50 (MQ=255)                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:3833031/1‑49 (MQ=255)                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:665676/48‑1 (MQ=255)                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              <  1:2663368/49‑1 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:4992590/50‑1 (MQ=255)                                  cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           >  1:4232564/1‑50 (MQ=255)                                   tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1599725/1‑50 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:337143/49‑1 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:2433495/50‑1 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:5342873/1‑47 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:5035885/1‑47 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAg       <  1:1223722/50‑1 (MQ=255)                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:5459767/1‑50 (MQ=255)                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:2782636/1‑50 (MQ=255)                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:3353366/1‑50 (MQ=255)                                           tCGGGTCTTCCAGCAGGCCGATTACGTTATTGCGATTATCGTCAGACtt   <  1:20702/49‑1 (MQ=38)                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:364516/1‑50 (MQ=255)                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:4838378/49‑1 (MQ=255)                                               |                                              CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑