| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,760 | A→G | N431S (AAC→AGC) | igaA → | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,760 | 0 | A | G | 98.5% | 263.6 / ‑3.7 | 66 | N431S (AAC→AGC) | igaA | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (47/18); total (47/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
AACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCG > NC_000913/3527713‑3527809 | aaCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcg < 1:1045321/50‑1 (MQ=255) aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga > 1:2540136/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:3823197/50‑1 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac < 1:862162/50‑1 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:1189083/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:4894823/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:430755/1‑50 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc > 1:5033630/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc > 1:5854921/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:4709297/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:5327845/50‑1 (MQ=255) tCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg > 1:5436361/1‑50 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc > 1:5594038/1‑50 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:3947703/1‑50 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt < 1:3414497/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:148563/1‑49 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa < 1:3601151/50‑1 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa < 1:5212021/50‑1 (MQ=255) cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa > 1:1990642/1‑50 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:101963/1‑50 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:5605319/1‑50 (MQ=255) tttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt > 1:5477586/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAtt < 1:3027596/49‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt < 1:5612096/48‑1 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg > 1:5414580/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:4332663/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:5288365/1‑50 (MQ=255) tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:4675259/1‑50 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa > 1:1305092/1‑50 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa < 1:693950/49‑1 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:3763786/1‑50 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:1510742/1‑50 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt < 1:4297985/49‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAcc < 1:1954773/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:2210508/1‑49 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:1364923/1‑48 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:746663/1‑48 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:4768715/1‑47 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:4447392/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAg < 1:5775697/49‑1 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:1365280/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:5056700/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:1476645/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa > 1:4037015/1‑50 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGa < 1:5921396/49‑1 (MQ=255) gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:3344180/1‑50 (MQ=255) gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:1771807/1‑50 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 1:4391137/49‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg < 1:4909961/50‑1 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:507041/1‑49 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:1000646/1‑49 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:5055820/1‑49 (MQ=255) cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa > 1:5259960/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGa < 1:2561296/48‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:5765532/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:4528236/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 1:2974165/50‑1 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt > 1:1757402/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt > 1:5324615/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:3494259/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg < 1:1681722/50‑1 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:5665420/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:5219585/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:475126/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg < 1:5194409/50‑1 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:5417167/1‑49 (MQ=255) tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:864541/1‑49 (MQ=255) catcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTc > 1:1510814/1‑49 (MQ=255) atcTGGCGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca < 1:1742261/49‑1 (MQ=38) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:4240758/1‑50 (MQ=255) tcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:3209831/1‑49 (MQ=255) tGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:266151/1‑49 (MQ=255) ggAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:1709299/1‑48 (MQ=255) acgacgCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCg < 1:4568119/50‑1 (MQ=255) | AACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCG > NC_000913/3527713‑3527809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |