Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,829,233	C→A	L97M (CTG→ATG)	xanP →	xanthine:H(+) symporter XanP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,829,233	0	C	A	100.0%	198.4 / NA	51	L97M (CTG→ATG)	xanP	xanthine:H(+) symporter XanP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/19);  total (32/19)

TGGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAT  >  NC_000913/3829186‑3829279                                                |                                               tgGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtg                                              <  1:5709543/50‑1 (MQ=255)   gCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgtt                                            <  1:1303014/50‑1 (MQ=255)   gCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgtt                                            <  1:3511942/50‑1 (MQ=255)   gCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgtt                                            <  1:2050797/50‑1 (MQ=255)   gCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgt                                             <  1:5891713/49‑1 (MQ=255)    cATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgttg                                           >  1:5473391/1‑50 (MQ=255)    cATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtgttg                                           <  1:2429769/50‑1 (MQ=255)      tCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTc                                         <  1:5223090/50‑1 (MQ=255)        gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCt                                        <  1:2583104/49‑1 (MQ=255)        gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCt                                        <  1:5679214/49‑1 (MQ=255)        gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTa                                       >  1:2061628/1‑50 (MQ=255)        gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTa                                       >  1:1714242/1‑50 (MQ=255)          ttattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAtt                                     <  1:4541310/50‑1 (MQ=255)          ttattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAtt                                     <  1:1335695/50‑1 (MQ=255)           tattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAtt                                     >  1:3203178/1‑49 (MQ=255)            attCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAtt                                     >  1:582308/1‑48 (MQ=255)               cAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAggg                                >  1:4445333/1‑50 (MQ=255)                aaaTTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAggg                                >  1:4982165/1‑49 (MQ=255)                   ttAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCAcc                            <  1:3463353/50‑1 (MQ=255)                      aGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGc                         >  1:5051603/1‑50 (MQ=255)                       ggCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCt                        <  1:1744614/50‑1 (MQ=255)                       ggCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCt                        <  1:3341000/50‑1 (MQ=255)                         ccTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCtt                       >  1:1923188/1‑49 (MQ=255)                         ccTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTc                      <  1:5046001/50‑1 (MQ=255)                          cTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTc                      >  1:4617432/1‑49 (MQ=255)                          cTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTc                      >  1:622910/1‑49 (MQ=255)                           tGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCaa                    >  1:2149137/1‑50 (MQ=255)                           tGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCaa                    >  1:4366407/1‑50 (MQ=255)                           tGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCaa                    >  1:1196156/1‑50 (MQ=255)                            ggggTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAAc                   >  1:2557058/1‑50 (MQ=255)                              ggTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACtt                 >  1:6026907/1‑50 (MQ=255)                               gTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACttt                <  1:401890/50‑1 (MQ=255)                               gTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACttt                <  1:1717367/50‑1 (MQ=255)                               gTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACttt                >  1:4690698/1‑50 (MQ=255)                                  cGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgtt             >  1:4226501/1‑50 (MQ=255)                                  cGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgtt             >  1:3808212/1‑50 (MQ=255)                                  cGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgtt             >  1:3773986/1‑50 (MQ=255)                                   ggTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttgttg            >  1:5318737/1‑50 (MQ=255)                                    gTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGc           >  1:980761/1‑50 (MQ=255)                                     ttGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcc          <  1:3447906/50‑1 (MQ=255)                                     ttGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcc          >  1:3353887/1‑50 (MQ=255)                                        gCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCg       >  1:2602043/1‑50 (MQ=255)                                         cTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGc      >  1:2953132/1‑50 (MQ=255)                                          tCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt     >  1:3567058/1‑50 (MQ=255)                                          tCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt     >  1:3244899/1‑50 (MQ=255)                                           ccGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt     >  1:3539643/1‑49 (MQ=255)                                           ccGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCt     >  1:5852149/1‑49 (MQ=255)                                            cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGa   >  1:931412/1‑50 (MQ=255)                                             gggATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAt  >  1:5566235/1‑50 (MQ=255)                                             gggATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAt  >  1:2818966/1‑50 (MQ=255)                                             gggATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAt  <  1:2542202/50‑1 (MQ=255)                                                |                                               TGGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAT  >  NC_000913/3829186‑3829279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑