BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,438,124	T→C	W140R (TGG→CGG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,438,124	0	T	C	100.0%	525.7 / NA	130	W140R (TGG→CGG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (68/62); total (68/62)

Only 100 of 130 total aligned reads displayed.

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

tCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:3823003/1‑50 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:2661347/1‑49 (MQ=255)

 cAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCgg                                             >  1:539832/1‑49 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  1:789603/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  1:1128023/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCggg                                            <  1:2317353/49‑1 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:6000641/1‑50 (MQ=255)

  aaaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           <  1:3736032/50‑1 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:1540835/1‑49 (MQ=255)

   aaaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGa                                           >  1:5760591/1‑49 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:2906444/1‑50 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:3030148/1‑50 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:5613009/1‑50 (MQ=255)

    aaaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGata                                         >  1:5223499/1‑50 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGTGatat                                        <  1:2852414/50‑1 (MQ=255)

     aaCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGatat                                        <  1:3236274/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       >  1:5776796/1‑50 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:2080618/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       >  1:5997277/1‑50 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:4283337/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATAtt                                       <  1:39325/50‑1 (MQ=255)

      aCCGTGAATTTCCGTTAGAACAAAACAGACGATTGAATGCCCGGGATAtt                                       <  1:5261120/50‑1 (MQ=255)

       ccGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      <  1:5043976/50‑1 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:2148928/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTc                                      >  1:583736/1‑49 (MQ=255)

        cGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCt                                     <  1:5778871/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:4286609/1‑50 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:3219188/1‑50 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    <  1:3083268/50‑1 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:1260051/1‑50 (MQ=255)

         gTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    <  1:1044558/50‑1 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:4087653/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:1842771/1‑49 (MQ=255)

          tGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTAc                                   <  1:842787/50‑1 (MQ=255)

           gAATTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:144109/1‑48 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTa                                    >  1:2520406/1‑47 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:5079815/49‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACa                                  <  1:5004147/49‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:825897/50‑1 (MQ=255)

            aaTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 <  1:219461/50‑1 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAg                                 >  1:5114840/1‑49 (MQ=255)

             aTTTCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGa                                <  1:5155977/50‑1 (MQ=255)

               ttCCGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaa                               >  1:3452985/1‑49 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           <  1:2885738/50‑1 (MQ=255)

                  cGTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaa                            <  1:2200787/49‑1 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:5352198/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:1488885/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:4055246/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:1270861/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:2912395/1‑49 (MQ=255)

                   gTTAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAAc                          <  1:5135672/50‑1 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:1972462/1‑48 (MQ=255)

                    ttAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGaaaaaa                           >  1:1093523/1‑48 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:231533/1‑50 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:2419405/1‑50 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:4303853/1‑50 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:2406975/1‑50 (MQ=255)

                     tAGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAg                        >  1:27799/1‑50 (MQ=255)

                      aGAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGt                       >  1:728317/1‑50 (MQ=255)

                       gAACAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTc                      <  1:5467980/50‑1 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     >  1:3264780/1‑50 (MQ=255)

                        aaCAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCt                     <  1:5257584/50‑1 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:5089576/1‑50 (MQ=255)

                          cAAAACAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   <  1:602953/50‑1 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGa                   >  1:5994744/1‑49 (MQ=255)

                           aaaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGAc                  <  1:694666/50‑1 (MQ=255)

                            aaaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:4451779/1‑50 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACa                 >  1:1280927/1‑49 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:4465473/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:4822827/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:2081079/50‑1 (MQ=255)

                             aaCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACAt                <  1:1659949/50‑1 (MQ=255)

                              aCAGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATa               >  1:1777147/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:3524853/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             >  1:4122924/1‑50 (MQ=255)

                                aGACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:1743383/50‑1 (MQ=255)

                                aGACGATTGTATACCCGGGATATTCTACAGAAAAAACAGTCTGACATAcc             <  1:5837922/50‑1 (MQ=255)

                                 gACGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            <  1:39390/50‑1 (MQ=255)

                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            >  1:4855757/1‑49 (MQ=255)

                                  aCGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCg            >  1:2666156/1‑49 (MQ=255)

                                   cGATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          <  1:468405/50‑1 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa         <  1:4011608/50‑1 (MQ=255)

                                    gATTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa          >  1:377974/1‑49 (MQ=255)

                                     aTTGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaaa        <  1:5909557/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  1:1855796/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  1:3778390/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:2111768/1‑50 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       <  1:4215576/50‑1 (MQ=255)

                                      ttGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:2291158/1‑50 (MQ=255)

                                       tGTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg       >  1:2768811/1‑49 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:3885484/1‑50 (MQ=255)

                                        gTATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     >  1:2579564/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:1665702/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:1666376/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa     <  1:1059178/49‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  1:3404924/50‑1 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    >  1:2395108/1‑50 (MQ=255)

                                         tATGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGAt    <  1:3120805/50‑1 (MQ=255)

                                           tGCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  <  1:714232/50‑1 (MQ=255)

                                            gCCCGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTa  >  1:3093931/1‑49 (MQ=255)

TCAAAAACCGTGAATTTCCGTTAGAACAAAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTA  >  NC_000913/4438077‑4438169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`