Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,013	0	G	.	100.0%	118.8 / NA	25	intergenic (‑132/+56)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (12/13);  total (12/13)

TGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379964‑380057                                                  |                                             tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:562002/1‑50 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:1591974/1‑50 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:187775/1‑50 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:371617/1‑50 (MQ=255)  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                             >  1:1359096/1‑50 (MQ=255)    agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                           <  1:1622833/50‑1 (MQ=255)      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                         <  1:447407/50‑1 (MQ=255)      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                         >  1:2475075/1‑50 (MQ=255)       aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg                                        >  1:1316581/1‑50 (MQ=255)        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                       >  1:3943408/1‑50 (MQ=255)         aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggg                                      >  1:185615/1‑50 (MQ=255)          aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggg                                      <  1:2681823/49‑1 (MQ=255)          aaaTTTATCCGGTGAATGTGGGCGGAAAACAAAGAGGAAAgggggggggc                                     <  1:787256/50‑2 (MQ=255)           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     >  1:2073872/1‑48 (MQ=255)           aaTTTATCCGGTGAATGTGGGCGGAAAACAAAGAGGAAAgggggggggc                                     >  1:3394314/1‑48 (MQ=255)            aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                    >  1:1734177/1‑47 (MQ=255)            aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                    >  1:1920676/1‑47 (MQ=255)              ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt                                >  1:2800057/1‑50 (MQ=255)                   cGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGc                            <  1:76747/49‑1 (MQ=255)                       gAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAggg                        >  1:3123103/1‑49 (MQ=255)                        aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGa                       <  1:2321443/49‑1 (MQ=255)                        aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGa                       <  1:2663807/49‑1 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     >  1:1330438/1‑50 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     <  1:2473672/50‑1 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     <  1:731371/50‑1 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     >  1:2487023/1‑50 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     <  1:2498121/50‑1 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAg                     <  1:2625464/50‑1 (MQ=255)                         aTGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAgg                    >  1:731333/1‑50 (MQ=255)                           gtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgc                   >  1:3892701/1‑50 (MQ=255)                            tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                  >  1:1356510/1‑50 (MQ=255)                             ggTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccg                 >  1:2891733/1‑50 (MQ=255)                                 ggAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc             >  1:1630205/1‑50 (MQ=255)                                 ggAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc             <  1:3024476/50‑1 (MQ=255)                                    aaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa          >  1:3779244/1‑50 (MQ=255)                                     aaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa          >  1:3307986/1‑49 (MQ=255)                                     aaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa          >  1:2881945/1‑49 (MQ=255)                                      aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa        <  1:3060204/50‑1 (MQ=255)                                       cAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg       <  1:2825366/50‑1 (MQ=255)                                        aaaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc      <  1:2668886/50‑1 (MQ=255)                                          agagGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg    <  1:2663602/50‑1 (MQ=255)                                            agGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc  <  1:544553/50‑1 (MQ=255)                                                  |                                             TGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379964‑380057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑