Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	171.0 / NA	36	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (13/23);  total (13/23)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234                                                  |                                              gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:2872658/1‑50 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               <  1:995550/50‑1 (MQ=255) gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               <  1:1658224/50‑1 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:2367550/1‑48 (MQ=255)     tttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:2879649/1‑47 (MQ=255)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        <  1:772885/49‑1 (MQ=38)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:3301490/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:3601468/50‑1 (MQ=39)            gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   >  1:3645037/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   <  1:1644426/49‑1 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:837948/1‑49 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:1294451/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:3300332/48‑1 (MQ=38)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1746849‑M1/1‑49 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1673732‑M1/1‑49 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             <  1:1245210‑M1/49‑1 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:3315281‑M1/1‑47 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTGATCGGTATTAtag                          >  1:920903‑M1/1‑46 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:3438071‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:2935575‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:28821‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:226364‑M1/50‑5 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:2576525‑M1/50‑6 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:1975179‑M1/1‑42 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    <  1:1756794‑M1/49‑10 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaa                   <  1:1819611‑M1/49‑11 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  <  1:591390‑M1/49‑12 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:3142581‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:1943818‑M1/49‑13 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaa             <  1:3726611‑M1/49‑17 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:599951‑M1/1‑29 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       <  1:203365‑M1/50‑23 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:3084789‑M1/48‑21 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacat      <  1:2521140‑M1/49‑24 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    <  1:1352661‑M1/50‑26 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    <  1:1904272‑M1/50‑26 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   >  1:1573090‑M1/1‑24 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   >  1:3511014‑M1/1‑24 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   >  1:2053215‑M1/1‑24 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   >  1:300262‑M1/1‑24 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  <  1:1604281‑M1/50‑28 (MQ=255)                                                  |                                              GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑