Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	195.5 / NA	49	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

TTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCA  >  NC_000913/12039‑12129                                                |                                            ttAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAg                                           >  1:1092046/1‑50 (MQ=255)   agtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                         >  1:239382/1‑50 (MQ=255)   agtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGt                                          <  1:476433/49‑1 (MQ=255)    gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                         >  1:686451/1‑49 (MQ=255)    gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                         <  1:481252/49‑1 (MQ=255)     tagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGa                                       <  1:3171497/50‑1 (MQ=255)        tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                    <  1:3260708/50‑1 (MQ=255)          aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAcc                                   <  1:308336/49‑1 (MQ=255)          aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                    >  1:1279041/1‑48 (MQ=255)          aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                    >  1:3727728/1‑48 (MQ=255)          aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCa                                  <  1:2270315/50‑1 (MQ=255)           aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                    >  1:1240780/1‑47 (MQ=255)           aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAc                                    >  1:2725482/1‑47 (MQ=255)           aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAg                                 <  1:1525595/50‑1 (MQ=255)           aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAg                                 <  1:284289/50‑1 (MQ=255)           aCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAg                                 <  1:283642/50‑1 (MQ=255)              gCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                              <  1:3444225/50‑1 (MQ=255)                agtgagtgAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGtt                            <  1:3292786/50‑1 (MQ=255)                 gtgagtgaGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGttt                           >  1:235808/1‑50 (MQ=255)                 gtgagtgaGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGttt                           >  1:1852320/1‑50 (MQ=255)                  tgagtgagTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                          <  1:2625948/50‑1 (MQ=255)                  tgagtgagTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                          <  1:2673694/50‑1 (MQ=255)                   gagtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                         >  1:3114666/1‑50 (MQ=255)                    agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGc                        >  1:539424/1‑50 (MQ=255)                    agtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGc                        >  1:997797/1‑50 (MQ=255)                     gtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcc                       >  1:1745311/1‑50 (MQ=255)                         gtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTa                   >  1:1834401/1‑50 (MQ=255)                            tGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:861883/1‑50 (MQ=255)                              cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:1797697/1‑48 (MQ=255)                              cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACa              <  1:950865/50‑1 (MQ=255)                               aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:2474874/1‑47 (MQ=255)                               aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:2427975/1‑47 (MQ=255)                               aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                >  1:241740/1‑47 (MQ=255)                                 aaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGAc           <  1:2095375/50‑1 (MQ=255)                                  aaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt          <  1:3503339/50‑1 (MQ=255)                                   aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt          >  1:2355599/1‑49 (MQ=255)                                   aaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACt          >  1:16650/1‑49 (MQ=255)                                    aaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca        <  1:1698798/50‑1 (MQ=255)                                      tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca      <  1:1265819/50‑1 (MQ=255)                                      tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca      <  1:237212/50‑1 (MQ=255)                                      tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca      <  1:3289289/50‑1 (MQ=255)                                      tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcac       >  1:846205/1‑49 (MQ=255)                                        aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa     <  1:3798292/49‑1 (MQ=255)                                        aaaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa     <  1:2702717/49‑1 (MQ=255)                                         aaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAcc   <  1:3364477/50‑1 (MQ=255)                                         aaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAc    >  1:2114838/1‑49 (MQ=255)                                          aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACca  >  1:413847/1‑50 (MQ=255)                                          aTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACca  >  1:506864/1‑50 (MQ=255)                                           ttGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACca  >  1:244741/1‑49 (MQ=255)                                                |                                            TTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCA  >  NC_000913/12039‑12129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑