BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	178.6 / NA	44	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/22); total (22/22)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:971841/1‑50 (MQ=255)

gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              <  1:2374423/50‑1 (MQ=255)

 cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             >  1:2516228/1‑50 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:3015444/1‑50 (MQ=255)

   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           <  1:3640590/50‑1 (MQ=255)

    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:1706053/1‑49 (MQ=255)

    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:3588977/1‑49 (MQ=255)

    aaCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           >  1:3513552/1‑49 (MQ=255)

      cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        <  1:651380/50‑1 (MQ=255)

      cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                        >  1:2427743/1‑50 (MQ=255)

          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:3144971/49‑1 (MQ=255)

          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                     <  1:699494/49‑1 (MQ=255)

             ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:934238/1‑48 (MQ=255)

               tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                >  1:887453/1‑49 (MQ=255)

                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  1:734748/48‑1 (MQ=255)

                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             >  1:1935285/1‑50 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          <  1:2732353/50‑1 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:275305/1‑50 (MQ=255)

                      tCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        >  1:468178/1‑50 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  1:2496060/48‑1 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:729951/49‑1 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:3750929/49‑1 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       >  1:1802384/1‑50 (MQ=255)

                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       <  1:1097192/49‑1 (MQ=255)

                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      >  1:1998565/1‑50 (MQ=255)

                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     <  1:3336617/50‑1 (MQ=255)

                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     >  1:1795942/1‑50 (MQ=255)

                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     <  1:1167992/50‑1 (MQ=255)

                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    >  1:474874/1‑50 (MQ=255)

                           aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:331952/1‑50 (MQ=255)

                           aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:2593007/1‑50 (MQ=255)

                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 <  1:3554189/49‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:2615252/1‑48 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:646826/1‑48 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  1:479836/50‑1 (MQ=255)

                                 tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              >  1:2926104/1‑49 (MQ=255)

                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           <  1:3366242/50‑1 (MQ=255)

                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:1117890/50‑1 (MQ=255)

                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          <  1:106064/50‑1 (MQ=255)

                                      tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa        <  1:3905484/50‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:1383745/49‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:3609543/49‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:2063854/49‑1 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:2836396/1‑50 (MQ=255)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`