Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	143.6 / NA	36	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/21);  total (15/21)

TTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTG  >  NC_000913/1154316‑1154408                                               |                                               tttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                             >  1:2613399/1‑50 (MQ=255)   tCAATGGCTGCTGCGTTTCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           >  1:2870882/1‑50 (MQ=255)   tCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           >  1:1010528/1‑50 (MQ=255)     aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                         >  1:3124848/1‑50 (MQ=255)      aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACtt                                        <  1:96913/50‑1 (MQ=255)        ggCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAc                                      >  1:3778051/1‑50 (MQ=255)         gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                     <  1:2361751/50‑1 (MQ=255)         gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                     <  1:3754267/50‑1 (MQ=255)         gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                     <  1:3188313/50‑1 (MQ=255)             ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 <  1:488691/50‑1 (MQ=255)              tgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 >  1:3197768/1‑49 (MQ=255)                cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCttt                                <  1:702078/48‑1 (MQ=255)                 gTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTaca                             <  1:483687/50‑1 (MQ=255)                    tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          <  1:2560213/50‑1 (MQ=255)                      gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:572692/1‑48 (MQ=255)                       aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:2213883/1‑47 (MQ=255)                        aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGa                      <  1:294086/50‑1 (MQ=255)                         ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                     >  1:3056786/1‑50 (MQ=255)                           ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                   <  1:1028381/50‑1 (MQ=255)                           ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                   >  1:2705151/1‑50 (MQ=255)                           ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                   >  1:1888826/1‑50 (MQ=255)                               cTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt               >  1:1546700/1‑50 (MQ=255)                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGGCCGTg              <  1:3110391/48‑1 (MQ=39)                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                 <  1:2468710/45‑1 (MQ=255)                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt               <  1:862886/47‑1 (MQ=255)                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc             <  1:2364676/49‑1 (MQ=255)                                   ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:3596894/1‑50 (MQ=255)                                   ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg            <  1:2922298/49‑1 (MQ=255)                                    tcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:134160/1‑49 (MQ=255)                                     cACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:3562007/1‑48 (MQ=255)                                      aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCga         <  1:1332292/49‑1 (MQ=255)                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt      <  1:1220587/48‑1 (MQ=255)                                           aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc    <  1:463444/49‑1 (MQ=255)                                           aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc    <  1:3704005/49‑1 (MQ=255)                                            aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt   <  1:3759136/49‑1 (MQ=255)                                            aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTg  <  1:3224760/50‑1 (MQ=255)                                               |                                               TTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTG  >  NC_000913/1154316‑1154408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑