Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	188.9 / NA	50	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/14);  total (36/14)

CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270764‑1270856                                                |                                              catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:778741/1‑50 (MQ=255) catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:1698456/1‑50 (MQ=255)  atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                             >  1:1372196/1‑49 (MQ=255)    caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:995916/1‑50 (MQ=255)    caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:1209528/1‑50 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                          >  1:2671221/1‑49 (MQ=255)     aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                         <  1:2890633/50‑1 (MQ=255)      aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                        <  1:2950269/50‑1 (MQ=255)       aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                       >  1:2728138/1‑50 (MQ=255)          ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                    <  1:391553/50‑1 (MQ=255)            cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg                                  >  1:1065278/1‑50 (MQ=255)             ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                 >  1:2289670/1‑50 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                >  1:1277137/1‑50 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                <  1:463868/50‑1 (MQ=255)              gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                >  1:2602370/1‑50 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:3195347/1‑50 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               <  1:2571068/50‑1 (MQ=255)               cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                               >  1:3645479/1‑50 (MQ=255)                 aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga                             <  1:3918006/50‑1 (MQ=255)                  aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                            <  1:993446/50‑1 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:68982/1‑50 (MQ=255)                     gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:2798916/1‑50 (MQ=255)                      tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg                        <  1:2978536/50‑1 (MQ=255)                        ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                         >  1:1988344/1‑47 (MQ=255)                           gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                   >  1:3630454/1‑50 (MQ=255)                            aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  >  1:1719668/1‑50 (MQ=255)                            aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                  >  1:1989845/1‑50 (MQ=255)                             tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                 <  1:3449047/50‑1 (MQ=255)                                gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:1272825/1‑50 (MQ=255)                                gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:336928/1‑50 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:527389/1‑48 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:1495666/1‑48 (MQ=255)                                  tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca              >  1:636125/1‑48 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca            <  1:3865456/48‑1 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg           >  1:2294812/1‑49 (MQ=255)                                    cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg           >  1:2218944/1‑49 (MQ=255)                                     gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa         >  1:2979331/1‑50 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:3694070/1‑50 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        <  1:2176638/50‑1 (MQ=255)                                      ccATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:820324/1‑50 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:446741/1‑49 (MQ=255)                                       cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc        >  1:102572/1‑49 (MQ=255)                                        aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt       >  1:1559221/1‑49 (MQ=255)                                         ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:368147/1‑49 (MQ=255)                                          tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:3121129/1‑48 (MQ=255)                                          tGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa      >  1:772846/1‑48 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa    >  1:2293100/1‑48 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:1889949/50‑1 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:1864315/50‑1 (MQ=255)                                            aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga  <  1:1352324/50‑1 (MQ=255)                                                |                                              CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGA  >  NC_000913/1270764‑1270856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑