Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	130.1 / NA	34	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)

ATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118982‑119074                                               |                                               aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             <  1:3543166/50‑1 (MQ=255)    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  1:3065356/49‑1 (MQ=255)    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  1:43364/49‑1 (MQ=255)    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctctc                                           <  1:1642716/49‑1 (MQ=255)     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         <  1:1797703/50‑1 (MQ=255)     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         <  1:3875416/50‑1 (MQ=255)       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       <  1:34843/50‑1 (MQ=255)       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  1:475828/1‑50 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:1385465/1‑50 (MQ=255)        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:173637/1‑50 (MQ=255)          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCa                                    <  1:2111541/50‑1 (MQ=255)            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  <  1:2170703/50‑1 (MQ=255)               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:3469014/1‑50 (MQ=255)                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:2096570/1‑50 (MQ=255)                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            <  1:733405/48‑1 (MQ=255)                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:2374665/49‑1 (MQ=255)                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:2060318/49‑1 (MQ=255)                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  1:1670059/1‑50 (MQ=255)                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  1:2811270/49‑1 (MQ=255)                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    >  1:3952811/1‑49 (MQ=255)                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:126553/1‑50 (MQ=255)                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:117434/1‑50 (MQ=255)                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  1:1172910/50‑1 (MQ=255)                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  1:820231/50‑1 (MQ=255)                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:2162574/1‑49 (MQ=255)                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  1:1774258/50‑1 (MQ=255)                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  1:2744978/50‑1 (MQ=255)                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:2351894/1‑50 (MQ=255)                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:860418/1‑50 (MQ=255)                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          >  1:3421296/1‑50 (MQ=255)                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttttt          <  1:1058849/50‑1 (MQ=255)                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:3244774/1‑49 (MQ=255)                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:1650606/1‑49 (MQ=255)                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:1416185/1‑49 (MQ=255)                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  1:2408180/49‑1 (MQ=255)                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  1:1488506/49‑1 (MQ=255)                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    >  1:102543/1‑50 (MQ=255)                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:3732093/50‑1 (MQ=255)                                               |                                               ATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118982‑119074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑