Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	194.4 / NA	49	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/29);  total (20/29)

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382508‑1382600                                                |                                              ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             >  1:780181/1‑50 (MQ=255)  tGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             >  1:168398/1‑49 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:1527267/48‑1 (MQ=255)   gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:2139555/48‑1 (MQ=255)    cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCa                                           <  1:3268005/49‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                          >  1:3097721/1‑49 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         <  1:3629408/50‑1 (MQ=255)     gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         <  1:2345547/50‑1 (MQ=255)      aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         >  1:2970778/1‑49 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                       <  1:1594120/50‑1 (MQ=255)       gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                       <  1:283625/50‑1 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                      >  1:2737467/1‑50 (MQ=255)        tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                      <  1:521734/50‑1 (MQ=255)          aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                    <  1:1216775/50‑1 (MQ=255)           aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:673694/1‑50 (MQ=255)            cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:1897446/1‑49 (MQ=255)             tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:126738/1‑48 (MQ=255)              attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                >  1:2783818/1‑50 (MQ=255)               ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                               <  1:646195/50‑1 (MQ=255)                   ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                           >  1:18539/1‑50 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                            <  1:2012257/46‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                            <  1:1172818/46‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                          <  1:1948624/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                          <  1:1405952/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                          <  1:2764323/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                          <  1:2517298/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                          <  1:2145628/48‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                           <  1:1959619/47‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                         <  1:3445140/49‑1 (MQ=255)                      gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                        <  1:1853777/50‑1 (MQ=255)                       aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                       >  1:3824915/1‑50 (MQ=255)                         cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                      >  1:1859390/1‑49 (MQ=255)                         cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCa                     <  1:3835851/50‑1 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   >  1:2916854/1‑49 (MQ=255)                            ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   >  1:2233558/1‑49 (MQ=255)                             gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaa                 <  1:631568/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                >  1:2851512/1‑50 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                <  1:2729504/50‑1 (MQ=255)                              cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                <  1:1572380/50‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt              <  1:572607/49‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt               <  1:3552720/48‑1 (MQ=255)                                 cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc             >  1:2268295/1‑50 (MQ=255)                                     atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         >  1:2200494/1‑50 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         <  1:3548336/49‑1 (MQ=255)                                      tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        >  1:3930889/1‑50 (MQ=255)                                       ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        >  1:278558/1‑49 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt     >  1:757142/1‑50 (MQ=255)                                         acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt     <  1:1207987/50‑1 (MQ=255)                                            acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg  <  1:595216/50‑1 (MQ=255)                                                |                                              TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382508‑1382600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑