| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 195,495 | A→G | E198G (GAG→GGG) | ispU → | undecaprenyl diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 195,495 | 0 | A | G | 82.5% | 97.6 / 14.3 | 40 | E198G (GAG→GGG) | ispU | undecaprenyl diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (16/17); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
TATGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGCC > NC_000913/195448‑195538 | tATGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGc < 1:762343/50‑1 (MQ=255) tGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCAt < 1:1007681/50‑1 (MQ=255) tGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCAt > 1:1498757/1‑50 (MQ=255) tGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCAt > 1:3206001/1‑50 (MQ=255) tGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCAt < 1:1773354/50‑1 (MQ=255) tGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCAt > 1:2529115/1‑50 (MQ=255) gCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATc > 1:2796124/1‑50 (MQ=255) cATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATc < 1:1186253/49‑1 (MQ=255) cATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCg > 1:1084811/1‑50 (MQ=255) aaCTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCAtt < 1:970204/50‑1 (MQ=255) aaCTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCAtt < 1:124151/50‑1 (MQ=255) aaCTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCAt > 1:3929534/1‑49 (MQ=255) aCTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTa < 1:3080563/50‑1 (MQ=255) aCTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGCATTa < 1:3691383/50‑1 (MQ=255) cTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAg > 1:732112/1‑50 (MQ=255) cTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAg > 1:3299404/1‑50 (MQ=255) gCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTa < 1:2793048/49‑1 (MQ=255) cccTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGCATTAGTAACt < 1:878140/50‑1 (MQ=255) cTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACttt < 1:2671002/50‑1 (MQ=255) cTGTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACttt > 1:3781795/1‑50 (MQ=255) gTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACttttt > 1:334138/1‑50 (MQ=255) gTAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACttttt > 1:1610452/1‑50 (MQ=255) tAGATTTTGTAATTAGGGCTGGGGGGGGGCATCGCATTAGTAACttttt < 1:33125/49‑1 (MQ=255) tAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTg < 1:2219481/50‑1 (MQ=255) tAGATTTAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTg > 1:2000117/1‑50 (MQ=255) gATTTAGTAATTAGGACTGGGGGGGAGCATCGCATTAGTAACTTTTTGc < 1:3193451/49‑1 (MQ=255) tttAGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCttt > 1:2524297/1‑50 (MQ=255) aGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTgg < 1:2608352/49‑1 (MQ=255) aGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTgg < 1:3587496/49‑1 (MQ=255) aGTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTgg < 1:536446/49‑1 (MQ=255) gTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTGGc > 1:3868480/1‑49 (MQ=255) gTAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTGGc < 1:2731404/49‑1 (MQ=255) tAATTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTGGCaa < 1:654797/50‑1 (MQ=255) aaTTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTGGCaaa > 1:3756964/1‑50 (MQ=255) aTTAGGACTGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTGGCAAAt < 1:2847476/50‑1 (MQ=255) aGGACTGGGGGGGAGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTg < 1:3071015/49‑1 (MQ=255) aGGACTGGGGGGGAGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGc > 1:3672015/1‑50 (MQ=255) cTGGGGGGGAGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGCCTAt > 1:2116672/1‑50 (MQ=255) tGGGGGGGGGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGCCTATg > 1:2984191/1‑50 (MQ=255) ggggggggCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGcc < 1:400624/50‑1 (MQ=255) ggggggAGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGc < 1:1308206/49‑1 (MQ=255) | TATGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGCATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGCC > NC_000913/195448‑195538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |