BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,412	C→T	R12H (CGT→CAT)	hycH ←	formate hydrogenlyase assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,412	0	C	T	100.0%	172.0 / NA	43	R12H (CGT→CAT)	hycH	formate hydrogenlyase assembly protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/21); total (22/21)

CCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGC  >  NC_000913/2843365‑2843457

ccTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATgg                                             <  1:2216618/50‑1 (MQ=255)

 ctgctgCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGc                                            <  1:856849/50‑1 (MQ=255)

   gctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                           >  1:3357359/1‑49 (MQ=255)

    ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                           <  1:3731122/48‑1 (MQ=255)

    ctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCa                                         <  1:1099529/50‑1 (MQ=255)

        gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGtt                                      >  1:2347541/1‑49 (MQ=255)

        gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                                     <  1:283443/50‑1 (MQ=255)

          cTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGAc                                   <  1:3268626/50‑1 (MQ=255)

           tCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                                  >  1:3816481/1‑50 (MQ=255)

             ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                                  <  1:2956240/48‑1 (MQ=255)

             ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTg                                 >  1:1567608/1‑49 (MQ=255)

              gcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa                               <  1:3821215/50‑1 (MQ=255)

              gcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGaa                               >  1:3748898/1‑50 (MQ=255)

                gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAaca                             <  1:109801/50‑1 (MQ=255)

                gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAaca                             >  1:2912038/1‑50 (MQ=255)

                 ggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac                            >  1:595088/1‑50 (MQ=255)

                  gcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac                            >  1:2352681/1‑49 (MQ=255)

                  gcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAacac                            >  1:1295499/1‑49 (MQ=255)

                   cgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcacca                          >  1:1942018/1‑50 (MQ=255)

                       gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt                      <  1:1591400/50‑1 (MQ=255)

                       gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCt                       <  1:614341/49‑1 (MQ=255)

                         aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                    >  1:3152194/1‑50 (MQ=255)

                          tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTc                   <  1:1893484/50‑1 (MQ=255)

                           ctttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                  >  1:1705517/3‑50 (MQ=255)

                           cGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                  >  1:1251896/1‑50 (MQ=255)

                            gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCa                  <  1:1797022/49‑1 (MQ=255)

                            gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc                 <  1:108080/50‑1 (MQ=255)

                            gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc                 >  1:2684115/1‑50 (MQ=255)

                            gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc                 >  1:3512076/1‑50 (MQ=255)

                             ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                >  1:2354869/1‑50 (MQ=255)

                             ttCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                >  1:2888300/1‑50 (MQ=255)

                              tctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                >  1:3416980/1‑49 (MQ=255)

                              tctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                >  1:2027365/1‑49 (MQ=255)

                                  atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa              <  1:2392022/47‑1 (MQ=255)

                                  atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATc            <  1:723806/49‑1 (MQ=255)

                                   tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCgg          <  1:482559/50‑1 (MQ=255)

                                    caATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa         >  1:3577953/1‑50 (MQ=255)

                                     aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa         <  1:2693593/49‑1 (MQ=255)

                                     aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGAt        <  1:509931/50‑1 (MQ=255)

                                       tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc      >  1:3518947/1‑50 (MQ=255)

                                       tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc      <  1:2063152/50‑1 (MQ=255)

                                          aTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg   <  1:770593/50‑1 (MQ=255)

                                           tttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACgcgc  >  1:3682154/1‑50 (MQ=255)

CCTGCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGC  >  NC_000913/2843365‑2843457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`