Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,898,938	T→C	I161V (ATC→GTC)	ygcU ←	putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,898,938	0	T	C	89.5%	115.6 / 6.3	38	I161V (ATC→GTC)	ygcU	putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (19/15);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTT  >  NC_000913/2898894‑2898981                                             |                                            gACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTc                                        >  1:3623860/1‑50 (MQ=255)   ccATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCgg                                      <  1:566492/50‑1 (MQ=255)   ccATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCgg                                      <  1:1869947/50‑1 (MQ=255)    cATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCggg                                     <  1:2858002/50‑1 (MQ=255)     atatCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGt                                    >  1:3032896/1‑50 (MQ=255)     atatCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGt                                    >  1:891540/1‑50 (MQ=255)     atatCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGt                                    >  1:2654490/1‑50 (MQ=255)          ttCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTa                               >  1:3567921/1‑50 (MQ=255)          ttCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTa                               >  1:2875766/1‑50 (MQ=255)            cGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCt                                <  1:1693155/47‑1 (MQ=255)            cGATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTAc                              <  1:1721378/49‑1 (MQ=255)             gATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTAcc                             <  1:2442746/49‑1 (MQ=255)             gATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTAc                              >  1:2844804/1‑48 (MQ=255)             gATTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCa                            <  1:1602906/50‑1 (MQ=255)              aTTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAg                           >  1:214441/1‑50 (MQ=255)              aTTGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAg                           <  1:3242891/50‑1 (MQ=255)               ttGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAgg                          >  1:2323511/1‑50 (MQ=255)                tGCGCCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGgc                         >  1:3082123/1‑50 (MQ=255)                 gcgcCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGgc                         <  1:481206/49‑1 (MQ=255)                  cgcCGTAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGgcc                        <  1:126966/49‑1 (MQ=255)                       tAGAGTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCAt                  <  1:481610/50‑1 (MQ=255)                        agagTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATc                 >  1:2834644/1‑50 (MQ=255)                        agagTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATc                 >  1:329539/1‑50 (MQ=255)                        agagTGTGGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATc                 >  1:3755221/1‑50 (MQ=255)                            tgtgGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTgcg             >  1:2002893/1‑50 (MQ=255)                             gtgGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTgcgc            >  1:2496841/1‑50 (MQ=255)                             gtgGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTgcgc            >  1:2196458/1‑50 (MQ=255)                              tgGAGAACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGcc           <  1:2634861/50‑1 (MQ=255)                                gagaACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCa          <  1:3598892/49‑1 (MQ=255)                                gagaACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAg         >  1:2193037/1‑50 (MQ=255)                                 agaACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGc        <  1:1842883/50‑1 (MQ=255)                                 agaACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGc        >  1:2130155/1‑50 (MQ=255)                                  gaACTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCg       >  1:2154759/1‑50 (MQ=255)                                    aCTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGc     <  1:275415/50‑1 (MQ=255)                                     cTGCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCt    <  1:3205399/50‑1 (MQ=255)                                       gCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCttt  >  1:71036/1‑50 (MQ=255)                                       gCCCGACACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCttt  >  1:1111850/1‑50 (MQ=255)                                        cccGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCtt   <  1:2616541/48‑1 (MQ=255)                                             |                                            GACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTT  >  NC_000913/2898894‑2898981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑