Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	262.2 / NA	65	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/41);  total (24/41)

GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477105‑3477195                                              |                                              gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:133752/1‑50 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           <  1:3021597/50‑1 (MQ=255) gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2543360/1‑50 (MQ=255)   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:649293/1‑48 (MQ=255)   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:3392123/1‑48 (MQ=255)   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2457956/1‑48 (MQ=255)   aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:633588/50‑1 (MQ=255)     gCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        >  1:33223/1‑49 (MQ=255)       ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      >  1:250721/1‑49 (MQ=255)         gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    <  1:3491825/49‑1 (MQ=255)          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   <  1:134440/49‑1 (MQ=255)          tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  <  1:3776438/50‑1 (MQ=255)           agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  >  1:3599894/1‑49 (MQ=255)           agagTCTATTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  >  1:447266/1‑49 (MQ=255)            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:1145113/49‑1 (MQ=255)            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:2331755/49‑1 (MQ=255)            gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                <  1:890228/50‑1 (MQ=255)             agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               <  1:587254/50‑1 (MQ=255)               tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             >  1:3704154/1‑50 (MQ=255)               tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:3141103/50‑1 (MQ=255)                  gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           >  1:2659071/1‑49 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:3348268/47‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         >  1:2055676/1‑50 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:876914/50‑1 (MQ=255)                   ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:3421315/50‑1 (MQ=255)                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       >  1:2038083/1‑50 (MQ=255)                     cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAaca                       >  1:392302/1‑50 (MQ=255)                      gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:1659091/1‑50 (MQ=255)                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:49762/1‑48 (MQ=255)                        tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:3675708/1‑48 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      <  1:3780074/44‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACAcc                     <  1:1397017/45‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:1196354/46‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:390619/47‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:206225/47‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:1639723/47‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:2100827/48‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:1190406/48‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:37092/48‑1 (MQ=255)                            ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGa                >  1:1022455/1‑50 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                 <  1:838403/47‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:3155767/49‑1 (MQ=255)                              ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:426763/1‑49 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:2391705/49‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:1681247/50‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:1838547/50‑1 (MQ=255)                               gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:3350450/50‑1 (MQ=255)                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:2814275/1‑50 (MQ=255)                                 cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:387346/1‑50 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           <  1:1420840/49‑1 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:2282264/1‑50 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:2555891/50‑1 (MQ=255)                                  ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:3101610/50‑1 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:1217210/50‑1 (MQ=255)                                   gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:863837/50‑1 (MQ=255)                                    agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        <  1:1669116/50‑1 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      <  1:1214242/50‑1 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      <  1:2582077/50‑1 (MQ=255)                                      agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      >  1:997239/1‑50 (MQ=255)                                       gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:2870281/50‑1 (MQ=255)                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:1261401/50‑1 (MQ=255)                                        tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    >  1:927919/1‑50 (MQ=255)                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:515114/49‑1 (MQ=255)                                          tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:3104466/49‑1 (MQ=255)                                           gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  <  1:1515933/49‑1 (MQ=255)                                              |                                              GAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477105‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑