Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	202.0 / NA	51	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/23);  total (28/23)

CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048                                               |                                              cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:1760254/1‑50 (MQ=255)  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:3522709/50‑1 (MQ=255)  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:323872/1‑50 (MQ=255)  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            <  1:2776347/49‑1 (MQ=255)   gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:3947077/1‑50 (MQ=255)    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:1337517/1‑49 (MQ=255)     gTCAGTGAGCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:2630398/50‑1 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:3542101/1‑48 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:998560/50‑1 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:2530960/50‑1 (MQ=255)      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:1544812/1‑50 (MQ=255)       cAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:1301149/1‑49 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:2375052/50‑1 (MQ=255)        aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:1733699/1‑48 (MQ=255)         gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  1:758778/50‑1 (MQ=255)          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:2732030/50‑1 (MQ=255)          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:2002661/1‑50 (MQ=255)           gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:1923020/1‑50 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:3728089/1‑50 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:3291615/50‑1 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  <  1:2044736/49‑1 (MQ=255)               gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              <  1:2593348/50‑1 (MQ=255)                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:927191/1‑49 (MQ=255)                cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:3446027/1‑49 (MQ=255)                  cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:1656728/50‑1 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:3921945/1‑50 (MQ=255)                   gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:966905/1‑50 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         <  1:617759/50‑1 (MQ=255)                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     <  1:1968532/50‑1 (MQ=255)                        aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:3333924/1‑50 (MQ=255)                          tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:2715668/1‑50 (MQ=255)                           ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:3901280/1‑49 (MQ=255)                             cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  1:341734/49‑1 (MQ=255)                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:1989072/1‑49 (MQ=255)                              ttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:3784603/1‑49 (MQ=255)                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:2650120/48‑1 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             <  1:382913/49‑1 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:3613846/50‑1 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            >  1:2730395/1‑50 (MQ=255)                                    aCCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:3871990/1‑49 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1600830/1‑48 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTc         <  1:380706/49‑1 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1769250/1‑47 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAg       <  1:3532453/50‑1 (MQ=255)                                      cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAg       <  1:1987910/50‑1 (MQ=255)                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:2820322/1‑50 (MQ=255)                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:2126783/1‑50 (MQ=255)                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:1344136/1‑50 (MQ=255)                                           tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:1071583/49‑1 (MQ=255)                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:3458110/1‑49 (MQ=255)                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  <  1:3074491/49‑1 (MQ=255)                                               |                                              CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑