| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,760 | A→G | N431S (AAC→AGC) | igaA → | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,760 | 0 | A | G | 98.0% | 198.9 / ‑3.5 | 51 | N431S (AAC→AGC) | igaA | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (35/15); total (35/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA > NC_000913/3527714‑3527804 | aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga < 1:2443525/50‑1 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 1:406956/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:1154072/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:1237320/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc > 1:2737812/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc > 1:1320988/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc > 1:512931/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc > 1:1468425/1‑50 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:1920886/50‑1 (MQ=255) aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:866091/1‑50 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:3481433/1‑49 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:2971300/50‑1 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:690603/50‑1 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:2795384/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg > 1:527310/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg > 1:2527070/1‑50 (MQ=255) ttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTg > 1:3531204/1‑50 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa > 1:1441639/1‑50 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAtt < 1:2614987/50‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:2900938/1‑49 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:2313805/1‑49 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt < 1:705664/49‑1 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa < 1:1403626/50‑1 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTa < 1:2350928/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3895817/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 1:1480144/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:776607/1‑50 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:611676/1‑49 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:275333/1‑49 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3317032/1‑48 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:1914027/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:654250/1‑47 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:2875497/1‑49 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:3691823/1‑49 (MQ=255) aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa < 1:683125/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:2939610/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:1729472/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt > 1:2241127/1‑50 (MQ=255) gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt > 1:1924192/1‑50 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 1:1219623/49‑1 (MQ=255) tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg > 1:1634892/1‑49 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa < 1:1297694/49‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:3078456/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:2368333/50‑1 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:1049587/50‑1 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:297723/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:765138/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:2337279/1‑50 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:656342/1‑50 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:3688354/1‑50 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca < 1:3332084/49‑1 (MQ=255) cTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaac > 1:1324772/1‑49 (MQ=255) ggAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:3443935/1‑48 (MQ=255) ggAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:3326134/1‑48 (MQ=255) ggAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:873528/1‑48 (MQ=255) | ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA > NC_000913/3527714‑3527804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |