Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	222.8 / NA	54	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

GGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988454‑988544                                              |                                              ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                           >  1:1885242/1‑50 (MQ=255)  gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                           >  1:4064116/1‑49 (MQ=255)  gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                          <  1:3515373/50‑1 (MQ=255)    aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                        <  1:1010198/50‑1 (MQ=255)     cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                       >  1:662846/1‑50 (MQ=255)     cATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaaa                                       >  1:3722684/1‑50 (MQ=255)      aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                      >  1:1363328/1‑50 (MQ=255)       tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     >  1:162693/1‑50 (MQ=255)         tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                    <  1:639085/49‑1 (MQ=255)          cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     >  1:504639/1‑47 (MQ=255)          cGCCTGAACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCa                                   <  1:3469565/49‑1 (MQ=39)           gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                 <  1:1769320/50‑1 (MQ=255)           gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                  <  1:3919154/49‑1 (MQ=255)            ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                <  1:2636421/50‑1 (MQ=255)            ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                <  1:1901570/50‑1 (MQ=255)              tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                               >  1:4193625/1‑49 (MQ=255)              tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                               >  1:3185412/1‑49 (MQ=255)                 aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                           <  1:1621671/50‑1 (MQ=255)                   cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                         <  1:4293915/50‑1 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                        <  1:1595681/48‑1 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                        <  1:1285355/48‑1 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                      >  1:3409396/1‑50 (MQ=255)                      cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                      >  1:793964/1‑50 (MQ=255)                       ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                     >  1:3401069/1‑50 (MQ=255)                        gTATCAGATGCGGTAATCAGTGACGTTGAAACACAGAAGAATCCCTGCTc                    >  1:2093083/1‑50 (MQ=255)                         tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                   <  1:2886133/50‑1 (MQ=255)                         tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                   >  1:2009103/1‑50 (MQ=255)                         tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                   <  1:3240051/50‑1 (MQ=255)                          aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  >  1:4135971/1‑50 (MQ=255)                           tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  <  1:3212664/49‑1 (MQ=255)                           tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  >  1:2065710/1‑49 (MQ=255)                            cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                 >  1:1172174/1‑49 (MQ=255)                            cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                 >  1:2751958/1‑49 (MQ=255)                            cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                 >  1:421195/1‑49 (MQ=255)                             aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaa               <  1:1776394/50‑1 (MQ=255)                             aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaa               <  1:2078761/50‑1 (MQ=255)                              gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa              <  1:2174772/50‑1 (MQ=255)                              gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                >  1:4131435/1‑48 (MQ=255)                               aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                >  1:2158579/1‑47 (MQ=255)                               aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag             <  1:2543956/50‑1 (MQ=255)                                tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga            <  1:2210363/50‑1 (MQ=255)                                tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag             >  1:1944599/1‑49 (MQ=255)                                tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag             >  1:3313996/1‑49 (MQ=255)                                  cGGTAATCAGTGACGTTGAACCCCAGAAGAATCCCTGCTCGTTAaagaa           <  1:3499585/49‑1 (MQ=255)                                   ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc         <  1:3358355/50‑1 (MQ=255)                                   ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc         >  1:4359720/1‑50 (MQ=255)                                    gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc         >  1:2889670/1‑49 (MQ=255)                                     tAATCAGTGACGTTGAAACCCAGGAGAATCCCTGCTCGTTAAAGAAGCGa       >  1:375178/1‑50 (MQ=255)                                     tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa       >  1:3826039/1‑50 (MQ=255)                                       aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt      <  1:754495/49‑1 (MQ=255)                                       aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg     >  1:2563635/1‑50 (MQ=255)                                        tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGc    <  1:1534061/50‑1 (MQ=255)                                          aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCa   <  1:3613515/49‑1 (MQ=255)                                          aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg  >  1:4290278/1‑50 (MQ=255)                                              |                                              GGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988454‑988544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑