Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	231.8 / NA	57	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/26);  total (31/26)

TCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270766‑1270857                                              |                                               tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:2572670/1‑48 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:1909723/1‑48 (MQ=255)   aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           >  1:699361/1‑49 (MQ=255)   aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg                                           <  1:1270867/49‑1 (MQ=255)   aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt                                          <  1:3623075/50‑1 (MQ=255)    aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:485630/50‑1 (MQ=255)     aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        >  1:3503505/1‑50 (MQ=255)     aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc                                        <  1:1000648/50‑1 (MQ=255)       aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:2685185/1‑50 (MQ=255)       aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt                                      >  1:1214948/1‑50 (MQ=255)        ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  1:832741/1‑50 (MQ=255)        ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     >  1:4073197/1‑50 (MQ=255)        ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc                                     <  1:683263/50‑1 (MQ=255)         gcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTcc                                    <  1:257171/50‑1 (MQ=255)           ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  1:1449268/1‑50 (MQ=255)           ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  >  1:598773/1‑50 (MQ=255)           ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg                                  <  1:2081459/50‑1 (MQ=255)             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:354268/50‑1 (MQ=255)             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:2538456/1‑50 (MQ=255)             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:2145499/1‑50 (MQ=255)             cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:898025/1‑50 (MQ=255)              aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg                               <  1:1960529/50‑1 (MQ=255)                 cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga                            <  1:2714876/50‑1 (MQ=255)                 cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag                             >  1:4199623/1‑49 (MQ=255)                  aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa                           <  1:1581013/50‑1 (MQ=255)                   gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:3846628/1‑50 (MQ=255)                     gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg                          >  1:3225569/1‑48 (MQ=255)                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag                       <  1:1922809/50‑1 (MQ=255)                      ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:3102826/49‑1 (MQ=255)                        aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  1:1769447/1‑49 (MQ=255)                        aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  1:3523771/1‑49 (MQ=255)                         gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata                    <  1:1532376/50‑1 (MQ=255)                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  1:758680/1‑50 (MQ=255)                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   >  1:1815788/1‑50 (MQ=255)                          aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat                   <  1:4362615/50‑1 (MQ=255)                           tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg                  <  1:4056280/50‑1 (MQ=255)                             cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg                 >  1:2890229/1‑49 (MQ=255)                              gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:2285035/1‑50 (MQ=255)                               ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:822175/1‑49 (MQ=255)                               ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:2229428/1‑49 (MQ=255)                               ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:2588186/1‑49 (MQ=255)                                tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:1117143/1‑48 (MQ=255)                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg            <  1:1823149/49‑1 (MQ=255)                                  cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  1:3846235/50‑1 (MQ=255)                                   gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          <  1:2042193/50‑1 (MQ=255)                                   gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa          >  1:1948105/1‑50 (MQ=255)                                     cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCAc         >  1:2904375/1‑49 (MQ=255)                                     cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:2166404/50‑1 (MQ=255)                                     cATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt        <  1:1668905/50‑1 (MQ=255)                                      aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:3625711/50‑1 (MQ=255)                                       ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa      <  1:4186537/50‑1 (MQ=255)                                       ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:3951774/1‑49 (MQ=255)                                         gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:2037087/1‑47 (MQ=255)                                          aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     >  1:4455261/1‑48 (MQ=255)                                          aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  1:393479/50‑1 (MQ=255)                                           gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:744754/50‑1 (MQ=255)                                           gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:3270327/50‑1 (MQ=255)                                              |                                               TCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270766‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑