Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	261.4 / NA	63	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/28);  total (35/28)

CGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382511‑1382601                                             |                                               cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:2289868/1‑50 (MQ=255) cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:3586264/1‑50 (MQ=255)  gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:570782/1‑49 (MQ=255)  gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:4356761/50‑1 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:2707305/1‑49 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:4093479/50‑1 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:161309/1‑50 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:2245003/50‑1 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        >  1:2496978/1‑50 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:2133889/50‑1 (MQ=255)    gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:3746915/50‑1 (MQ=255)     tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       >  1:3863236/1‑50 (MQ=255)     tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:4346888/50‑1 (MQ=255)       aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                     >  1:940149/1‑50 (MQ=255)         cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:3059794/1‑49 (MQ=255)         cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                   <  1:1000767/50‑1 (MQ=255)           attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 >  1:1442652/1‑50 (MQ=255)            ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                <  1:130471/50‑1 (MQ=255)            ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                                <  1:1131335/50‑1 (MQ=255)             tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               >  1:1816585/1‑50 (MQ=255)             tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               >  1:282042/1‑50 (MQ=255)             tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               >  1:2367372/1‑50 (MQ=255)              atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              >  1:2191236/1‑50 (MQ=255)              atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                               <  1:1397525/49‑1 (MQ=255)                ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            >  1:2643149/1‑50 (MQ=255)                ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            >  1:2505512/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTcc                           <  1:2381950/48‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:3903862/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:3892637/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:3605796/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         <  1:3600972/50‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAATCGCAATAACGGTGAGTTTTcc                           <  1:1419925/48‑1 (MQ=38)                    aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:3094849/50‑1 (MQ=255)                      cgcgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                       >  1:2814973/1‑49 (MQ=255)                        cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:3347565/50‑1 (MQ=255)                         ttcTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    >  1:3931743/3‑49 (MQ=255)                         ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    >  1:4224693/1‑49 (MQ=255)                          gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaa                  <  1:4450059/50‑1 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 <  1:2375986/50‑1 (MQ=255)                           cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                 >  1:3322769/1‑50 (MQ=255)                            ttCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                >  1:2997689/1‑50 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:96835/49‑1 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAtt               <  1:1000572/49‑1 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:2164783/48‑1 (MQ=255)                              cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              >  1:3258527/1‑50 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:2285468/49‑1 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:1592376/49‑1 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:1357049/49‑1 (MQ=255)                                atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            <  1:3545225/50‑1 (MQ=255)                                atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc            <  1:62930/50‑1 (MQ=255)                                  atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:949799/1‑50 (MQ=255)                                  atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:1948608/1‑50 (MQ=255)                                  atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:2030045/1‑50 (MQ=255)                                  atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc          >  1:1405117/1‑50 (MQ=255)                                     gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       >  1:151836/1‑50 (MQ=255)                                     gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTa       >  1:1028440/1‑50 (MQ=255)                                      acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      >  1:2093679/1‑50 (MQ=255)                                        gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    >  1:3111416/1‑50 (MQ=255)                                        gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:119515/50‑1 (MQ=255)                                         acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   >  1:2031785/1‑50 (MQ=255)                                         acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   >  1:2991463/1‑50 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:3085091/50‑1 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:1780787/50‑1 (MQ=255)                                             |                                               CGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382511‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑