BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	240.0 / NA	58	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/31); total (27/31)

GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTC  >  NC_000913/1697170‑1697262

gTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtga                                             >  1:2264711/1‑50 (MQ=255)

gTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtg                                              <  1:210705/49‑1 (MQ=255)

 ttAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                            >  1:3643712/1‑50 (MQ=255)

 ttAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                            >  1:3407909/1‑50 (MQ=255)

  tAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtg                                              >  1:1166465/1‑47 (MQ=255)

   aaCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                            >  1:573883/1‑48 (MQ=255)

    aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgatga                                          <  1:1884968/49‑1 (MQ=255)

    aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAg                                         <  1:2622830/50‑1 (MQ=255)

    aCCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAg                                         >  1:3244965/1‑50 (MQ=255)

     ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGCTGAGt                                        <  1:1951431/50‑1 (MQ=39)

     ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                        >  1:167229/1‑50 (MQ=255)

     ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                        <  1:4100906/50‑1 (MQ=255)

     ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                        >  1:1535649/1‑50 (MQ=255)

      cAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                       >  1:2799939/1‑50 (MQ=255)

       aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                       >  1:2143995/1‑49 (MQ=255)

        aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                     >  1:3681112/1‑50 (MQ=255)

        aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                     <  1:667579/50‑1 (MQ=255)

         tAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                    <  1:916818/50‑1 (MQ=255)

          aGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATc                                   >  1:3576056/1‑50 (MQ=255)

           gCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATcc                                  <  1:4030383/50‑1 (MQ=255)

            ccGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                                 <  1:3420975/50‑1 (MQ=255)

            ccGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                                 >  1:3702463/1‑50 (MQ=255)

            ccGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                                 <  1:447877/50‑1 (MQ=255)

             cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                <  1:3905426/50‑1 (MQ=255)

               tGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCgg                               >  1:3919027/1‑49 (MQ=255)

                gATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGt                              >  1:1898927/1‑49 (MQ=255)

                 aTTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaa                            <  1:3312565/50‑1 (MQ=255)

                   ttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                           >  1:1312519/1‑49 (MQ=255)

                    tGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                          <  1:2846043/49‑1 (MQ=255)

                     ggCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaaca                         <  1:282193/49‑1 (MQ=255)

                     ggCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaaca                         <  1:2402308/49‑1 (MQ=255)

                      gCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaacaa                        <  1:2310500/49‑1 (MQ=255)

                       cTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAacaaca                      >  1:560074/1‑50 (MQ=255)

                        tGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                     >  1:2260062/1‑50 (MQ=255)

                        tGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                     >  1:1062434/1‑50 (MQ=255)

                         gCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                    >  1:1635030/1‑50 (MQ=255)

                          ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacg                    <  1:218640/49‑1 (MQ=255)

                           cAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgca                  <  1:1799004/50‑1 (MQ=255)

                            aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 >  1:2119603/1‑50 (MQ=255)

                            aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 <  1:4187867/50‑1 (MQ=255)

                            aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 <  1:3155639/50‑1 (MQ=255)

                             gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcacg                >  1:332528/1‑50 (MQ=255)

                             gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcacg                >  1:1682196/1‑50 (MQ=255)

                                 tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg            <  1:3140017/50‑1 (MQ=255)

                                 tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg            <  1:3310162/50‑1 (MQ=255)

                                 tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttg            <  1:347137/50‑1 (MQ=255)

                                   tAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt          >  1:1671990/1‑50 (MQ=255)

                                    aGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt          <  1:3721681/49‑1 (MQ=255)

                                     gCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGttt         <  1:1594314/49‑1 (MQ=255)

                                      cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc       >  1:448553/1‑50 (MQ=255)

                                      cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc       <  1:777430/50‑1 (MQ=255)

                                       agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc      <  1:3765337/50‑1 (MQ=255)

                                       agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc      <  1:1595156/50‑1 (MQ=255)

                                        gagaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCg     <  1:2863154/50‑1 (MQ=255)

                                         agaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCgg    >  1:3512401/1‑50 (MQ=255)

                                          gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt   <  1:2995786/50‑1 (MQ=255)

                                          gaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGt   >  1:132581/1‑50 (MQ=255)

                                           aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTc  <  1:1876896/50‑1 (MQ=255)

GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTC  >  NC_000913/1697170‑1697262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`