Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	237.3 / NA	60	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/27);  total (33/27)

GCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCT  >  NC_000913/1811161‑1811253                                                |                                              gcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTg                                             >  1:3723682/1‑50 (MQ=255) gcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTg                                             >  1:2429167/1‑50 (MQ=255)  cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGc                                            >  1:2650492/1‑50 (MQ=255)  cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGc                                            >  1:29832/1‑50 (MQ=255)  cAAATGATCGTTATGCCATTAGTCTTCGCCTCTATTCTGAGCGCGGCTg                                             <  1:3148421/49‑1 (MQ=38)   aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                           <  1:4199183/50‑1 (MQ=255)   aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                           >  1:3933701/1‑50 (MQ=255)    aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGccc                                          <  1:530881/50‑1 (MQ=255)      tGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGt                                        >  1:2824441/1‑50 (MQ=255)       gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                       >  1:2227927/1‑50 (MQ=255)        aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                      <  1:172021/50‑1 (MQ=255)        aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                      >  1:2141260/1‑50 (MQ=255)          cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                     >  1:480549/1‑49 (MQ=255)           gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                   >  1:3472098/1‑50 (MQ=255)            ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  <  1:651277/50‑1 (MQ=255)            ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  <  1:593842/50‑1 (MQ=255)             tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  >  1:2704321/1‑49 (MQ=255)             tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                  >  1:3821864/1‑49 (MQ=255)              aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                >  1:3163113/1‑50 (MQ=255)              aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                 <  1:1071656/49‑1 (MQ=255)              aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                 <  1:647350/49‑1 (MQ=255)               tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                               >  1:179495/1‑50 (MQ=255)                 ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                              >  1:1874706/1‑49 (MQ=255)                 ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGc                             <  1:2864870/50‑1 (MQ=255)                   gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                           >  1:2223249/1‑50 (MQ=255)                   gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                           <  1:2821009/50‑1 (MQ=255)                     tAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                         >  1:4119445/1‑50 (MQ=255)                     tAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                         >  1:3513421/1‑50 (MQ=255)                      aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                         >  1:1812170/1‑49 (MQ=255)                      aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                         >  1:2764833/1‑49 (MQ=255)                      aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                         >  1:555922/1‑49 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:3108037/49‑1 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                        <  1:1687455/49‑1 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCa                       <  1:562994/50‑1 (MQ=255)                          ttCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                     >  1:638025/1‑49 (MQ=255)                            cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                    <  1:1811797/48‑1 (MQ=255)                            cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                     <  1:2021953/47‑1 (MQ=255)                            cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                  >  1:2053214/1‑50 (MQ=255)                             gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 <  1:1934172/50‑1 (MQ=255)                             gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 <  1:1017389/50‑1 (MQ=255)                              ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                 >  1:47546/1‑49 (MQ=255)                              ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                <  1:847487/50‑1 (MQ=255)                              ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                <  1:3780436/50‑1 (MQ=255)                                tctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa               >  1:1280505/1‑49 (MQ=255)                                tctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa               >  1:2985837/1‑49 (MQ=255)                                 ctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa              >  1:3723698/1‑49 (MQ=255)                                  tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa            >  1:432080/1‑50 (MQ=255)                                  tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa              <  1:2012363/48‑1 (MQ=255)                                   aTTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt           >  1:2497964/1‑50 (MQ=255)                                   aTTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt           >  1:523124/1‑50 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa            <  1:4123689/48‑1 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaa             <  1:4213887/47‑1 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt           >  1:2577412/1‑49 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATc          <  1:1374789/50‑1 (MQ=255)                                       tGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt       >  1:1889812/1‑50 (MQ=255)                                       tGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt       >  1:1141351/1‑50 (MQ=255)                                         aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGttt     <  1:4107276/50‑1 (MQ=255)                                         aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt      <  1:2571916/49‑1 (MQ=255)                                           cgcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTc   <  1:2248410/50‑1 (MQ=255)                                            gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt  <  1:3124233/50‑1 (MQ=255)                                                |                                              GCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCT  >  NC_000913/1811161‑1811253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑