Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	267.5 / NA	64	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/31);  total (33/31)

GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214839‑214931                                               |                                               gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:1324326/1‑50 (MQ=255)  tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             <  1:809843/49‑1 (MQ=255)  tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:3345005/1‑49 (MQ=255)    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          <  1:2155582/50‑1 (MQ=255)    aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          >  1:1711685/1‑50 (MQ=255)     tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                         <  1:3747379/50‑1 (MQ=255)      ttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        >  1:1019826/1‑50 (MQ=255)       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        <  1:4336672/49‑1 (MQ=255)       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       >  1:4405549/1‑50 (MQ=255)       tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       >  1:342188/1‑50 (MQ=255)        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       <  1:545306/49‑1 (MQ=255)        cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                      >  1:979893/1‑50 (MQ=255)         ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                      <  1:4263076/49‑1 (MQ=255)         ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:1674800/50‑1 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  1:3311823/50‑1 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  1:4005665/50‑1 (MQ=255)          cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    >  1:2216049/1‑50 (MQ=255)            aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:4392029/1‑49 (MQ=255)            aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:882773/1‑49 (MQ=255)              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                >  1:460593/1‑50 (MQ=255)              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                >  1:1432759/1‑50 (MQ=255)              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                <  1:1722423/50‑1 (MQ=255)              aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                >  1:1764707/1‑50 (MQ=255)               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:2248531/1‑50 (MQ=255)               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  1:1160470/50‑1 (MQ=255)               tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:3946512/1‑50 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             <  1:816209/50‑1 (MQ=255)                 ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             >  1:744715/1‑50 (MQ=255)                   gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           >  1:2438273/1‑50 (MQ=255)                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           <  1:2965985/49‑1 (MQ=255)                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          >  1:503891/1‑50 (MQ=255)                    cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          <  1:2443673/50‑1 (MQ=255)                     tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  1:2783906/1‑50 (MQ=255)                       ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       <  1:2487977/50‑1 (MQ=255)                          ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     <  1:2651618/49‑1 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:3298863/1‑49 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:1172992/1‑49 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:2331403/1‑49 (MQ=255)                           cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc                   <  1:2712281/50‑1 (MQ=255)                            gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:3094290/1‑48 (MQ=255)                            gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:2312119/1‑48 (MQ=255)                              tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagc                <  1:1975418/50‑1 (MQ=255)                              tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  1:4062761/1‑49 (MQ=255)                               ggTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca               >  1:34158/1‑50 (MQ=255)                                gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt              <  1:2735808/50‑1 (MQ=255)                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             >  1:2680318/1‑50 (MQ=255)                                 tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:59758/50‑1 (MQ=255)                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  1:1926473/49‑1 (MQ=255)                                   aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:2133983/1‑49 (MQ=255)                                       tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  1:450712/1‑49 (MQ=255)                                       tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  1:1966072/1‑49 (MQ=255)                                       tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  1:1587098/1‑49 (MQ=255)                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  1:2481411/49‑1 (MQ=255)                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  1:1456923/49‑1 (MQ=255)                                        gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        <  1:986105/48‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  1:729872/49‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  1:1724278/49‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg     <  1:598867/50‑1 (MQ=255)                                         agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg     <  1:1071807/50‑1 (MQ=255)                                          gaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGa    <  1:414550/50‑1 (MQ=255)                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   >  1:3764562/1‑50 (MQ=255)                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   >  1:236384/1‑50 (MQ=255)                                           atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   >  1:2029685/1‑50 (MQ=255)                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  >  1:4020026/1‑50 (MQ=255)                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  >  1:3181502/1‑50 (MQ=255)                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  <  1:2533552/50‑1 (MQ=255)                                            tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  <  1:1829011/50‑1 (MQ=255)                                               |                                               GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214839‑214931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑