Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,843,412	C→T	R12H (CGT→CAT)	hycH ←	formate hydrogenlyase assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,843,412	0	C	T	100.0%	148.7 / NA	37	R12H (CGT→CAT)	hycH	formate hydrogenlyase assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

GCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGCC  >  NC_000913/2843368‑2843458                                             |                                               gctgcGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCt                                            >  1:695166/1‑49 (MQ=255)     cgcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAg                                         <  1:3256309/48‑1 (MQ=255)      gcCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTg                                      <  1:3614270/50‑1 (MQ=255)       ccTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGa                                     >  1:3199278/1‑50 (MQ=255)           ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACt                                   <  1:3454226/48‑1 (MQ=255)           ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTg                                  >  1:12104/1‑49 (MQ=255)           ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTg                                  <  1:360135/49‑1 (MQ=255)           ggcgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTg                                  <  1:1711193/49‑1 (MQ=255)             cgggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAac                               <  1:1815570/50‑1 (MQ=255)              gggcgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAaca                              >  1:2834351/1‑50 (MQ=255)                 cgTGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAAcacca                           >  1:3751229/1‑50 (MQ=255)                   tGGCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACaccacc                         >  1:1275495/1‑50 (MQ=255)                     gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtt                       >  1:2345290/1‑50 (MQ=255)                     gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCt                        <  1:2332897/49‑1 (MQ=255)                     gCATCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCt                        <  1:2778062/49‑1 (MQ=255)                       aTCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                     <  1:221617/50‑1 (MQ=255)                        tCGTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCtttt                     >  1:4047429/1‑49 (MQ=255)                          gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc                  >  1:1893173/1‑50 (MQ=255)                          gTTCTCATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCAc                  >  1:1134731/1‑50 (MQ=255)                            tctcATCAATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACt                 >  1:305137/1‑49 (MQ=255)                                atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa               <  1:3224974/47‑1 (MQ=255)                                atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCa               <  1:395441/47‑1 (MQ=255)                                atcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg            <  1:13917/50‑1 (MQ=255)                                 tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCgg           <  1:1925381/50‑1 (MQ=255)                                 tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCgg           <  1:3449553/50‑1 (MQ=255)                                 tcaATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCg            >  1:2074886/1‑49 (MQ=255)                                  caATAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGa          >  1:2359207/1‑50 (MQ=255)                                   aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGAt         <  1:2473443/50‑1 (MQ=255)                                   aaTAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGAt         <  1:234762/50‑1 (MQ=255)                                     tAAATTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAc       >  1:4355397/1‑50 (MQ=255)                                      aaaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcg      >  1:2730098/1‑50 (MQ=255)                                       aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATTGGATAcgc     <  1:1807687/50‑1 (MQ=255)                                       aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgc     <  1:180583/50‑1 (MQ=255)                                       aaTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcg      <  1:4270114/49‑1 (MQ=255)                                        aTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg    >  1:3597767/1‑50 (MQ=255)                                        aTTTATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATAcgcg    >  1:2829742/1‑50 (MQ=255)                                          ttATGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGcc  >  1:273997/1‑50 (MQ=255)                                             |                                               GCTGCGCCTCGGCGGGCGTGGCATCGTTCTCATCAATAAATTTACGGCTCAGTTGACTGAACACCACCTTTTCACTCATCGGATACGCGCC  >  NC_000913/2843368‑2843458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑