Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,213,803	T→C	S253P (TCT→CCT)	rpoD →	RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,213,803	0	T	C	100.0%	215.9 / NA	53	S253P (TCT→CCT)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

GAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACT  >  NC_000913/3213757‑3213848                                               |                                              gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCt                                             >  1:4367783/1‑49 (MQ=255) gAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTg                                            <  1:1954820/50‑1 (MQ=255)  aaaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTg                                            >  1:3194812/1‑49 (MQ=255)   aaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTg                                            >  1:1234230/1‑48 (MQ=255)   aaGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGaa                                          <  1:1184744/50‑1 (MQ=255)    aGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAg                                         <  1:2157231/50‑1 (MQ=255)    aGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAg                                         <  1:1174101/50‑1 (MQ=255)     ggTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGt                                        <  1:1413837/50‑1 (MQ=255)     ggTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGt                                        <  1:1004366/50‑1 (MQ=255)     ggTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGt                                        <  1:1980602/50‑1 (MQ=255)      gTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTa                                       >  1:1434509/1‑50 (MQ=255)       tCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAt                                      <  1:951471/50‑1 (MQ=255)         gCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTAtt                                     <  1:3346459/49‑1 (MQ=255)          cAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTc                                    >  1:1967962/1‑49 (MQ=255)           aGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaa                                  <  1:2392363/50‑1 (MQ=255)             tCACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAAc                                <  1:3174294/50‑1 (MQ=255)              cACGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCaaa                                 >  1:2633844/1‑48 (MQ=255)               aCGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACa                               <  1:2570280/49‑1 (MQ=255)                cGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGt                             <  1:2317790/50‑1 (MQ=255)                cGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGt                             <  1:2284795/50‑1 (MQ=255)                cGCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGt                             <  1:998529/50‑1 (MQ=255)                 gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt                            <  1:4147285/50‑1 (MQ=255)                 gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt                            <  1:1684192/50‑1 (MQ=255)                 gCTACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGtt                            >  1:1574023/1‑50 (MQ=255)                   tACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc                          >  1:4451076/1‑50 (MQ=255)                   tACCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTcc                          >  1:2519617/1‑50 (MQ=255)                    aCCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCg                         >  1:2467073/1‑50 (MQ=255)                    aCCGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCg                         >  1:4058578/1‑50 (MQ=255)                      cGCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGcc                       >  1:77917/1‑50 (MQ=255)                       gCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCt                      <  1:4041265/50‑1 (MQ=255)                       gCTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCt                      >  1:3848583/1‑50 (MQ=255)                        cTCAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTg                     >  1:600255/1‑50 (MQ=255)                          cAGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGt                   >  1:3772301/1‑50 (MQ=255)                           aGGAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTg                  <  1:3829058/50‑1 (MQ=255)                            ggAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGc                 >  1:2062149/1‑50 (MQ=255)                            ggAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGc                 >  1:3604969/1‑50 (MQ=255)                             gAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc                >  1:1304835/1‑50 (MQ=255)                             gAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc                <  1:1696946/50‑1 (MQ=255)                             gAAGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGcc                <  1:1851216/50‑1 (MQ=255)                              aaGAGATCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg               <  1:1597158/50‑1 (MQ=255)                                gagaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCg               >  1:145799/1‑48 (MQ=255)                                gagaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGag             >  1:304406/1‑49 (MQ=255)                                gagaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGaa             <  1:3181654/50‑1 (MQ=255)                                 agaTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg            >  1:3675452/1‑50 (MQ=255)                                   aTCCTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAg            >  1:153878/1‑48 (MQ=255)                                      cTGAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGtt       <  1:3236517/50‑1 (MQ=255)                                        gAAACTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGttt      >  1:1943714/1‑49 (MQ=255)                                         aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTg     <  1:3925376/49‑1 (MQ=255)                                         aaaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGa    >  1:3069423/1‑50 (MQ=255)                                          aaCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGAc   <  1:1489990/50‑1 (MQ=255)                                           aCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGAc   >  1:4298802/1‑49 (MQ=255)                                           aCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACt  <  1:230088/50‑1 (MQ=255)                                           aCTGCCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACt  <  1:2050148/50‑1 (MQ=255)                                               |                                              GAAAGGTCGCAGTCACGCTACCGCTCAGGAAGAGATCCTGAAACTGTCTGAAGTATTCAAACAGTTCCGCCTGGTGCCGAAGCAGTTTGACT  >  NC_000913/3213757‑3213848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑