Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	289.5 / NA	71	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/51);  total (20/51)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195                                               |                                              cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:3741341/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:577721/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:800775/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            >  1:3978486/1‑50 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            >  1:4429059/1‑50 (MQ=255)  gAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           <  1:3244829/50‑1 (MQ=255)   aaTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATc                                          <  1:3711384/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2703635/1‑48 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTAt                                           >  1:2237195/1‑48 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:2378704/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:810856/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:4176275/50‑1 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:1296659/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        >  1:707588/1‑50 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        <  1:821455/50‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                       <  1:359469/49‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      <  1:2159936/50‑1 (MQ=255)        ttGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      >  1:4263675/1‑49 (MQ=255)         tGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaaa                                    >  1:3692849/1‑50 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAtt                                  <  1:1755641/50‑2 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   <  1:2381994/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   <  1:2410510/49‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  <  1:1106264/50‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  <  1:3729671/50‑1 (MQ=255)           tAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  <  1:539879/50‑1 (MQ=255)             gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGc                                 <  1:3322262/49‑1 (MQ=255)             gagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCa                                >  1:885874/1‑50 (MQ=255)              agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:2153672/1‑50 (MQ=255)              agTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCAt                               >  1:3411902/1‑50 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:719688/50‑1 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:3239704/50‑1 (MQ=255)                   gTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           >  1:2768996/1‑49 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           <  1:3217053/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           <  1:4297687/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTg                           <  1:1839733/48‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:3041499/50‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:2696792/50‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGa                          <  1:2951516/49‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:2330586/49‑1 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:2542601/1‑50 (MQ=255)                       gATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:517473/1‑50 (MQ=255)                         tctcTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:1481965/1‑48 (MQ=255)                           tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:861427/48‑1 (MQ=255)                           tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:777708/1‑50 (MQ=255)                           tctTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  >  1:3353518/1‑50 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCa                    <  1:2423158/46‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:508417/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:3410062/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:1297003/47‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:2452785/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:2063722/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:3303386/48‑1 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGc                  <  1:2912245/48‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                 <  1:747565/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               <  1:2457879/49‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:2384959/1‑49 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:2386562/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:1867096/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATc              <  1:2311986/49‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:439442/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:3359046/50‑1 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCa             <  1:2706031/50‑1 (MQ=255)                                 tCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAg            <  1:606137/50‑1 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          >  1:1071188/1‑50 (MQ=255)                                      gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:728234/50‑1 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      <  1:2121123/50‑1 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATc      >  1:988313/1‑50 (MQ=255)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     >  1:2519402/1‑50 (MQ=255)                                         tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:2278897/50‑1 (MQ=255)                                         tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:37807/50‑1 (MQ=255)                                         tttGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCtt    <  1:3206777/50‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:2896893/49‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:3324358/49‑1 (MQ=255)                                            gCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  <  1:2129903/49‑1 (MQ=255)                                               |                                              CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑