| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,760 | A→G | N431S (AAC→AGC) | igaA → | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,760 | 0 | A | G | 94.8% | 217.6 / 2.0 | 58 | N431S (AAC→AGC) | igaA | inner membrane protein ‑ inhibits the Rcs signaling pathway |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (36/19); total (38/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01 | |||||||||||
ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA > NC_000913/3527714‑3527804 | aCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcga > 1:1211209/1‑50 (MQ=255) ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac > 1:2700724/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg < 1:834486/50‑1 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:466621/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg < 1:3805088/50‑1 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:2969213/1‑50 (MQ=255) cAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgacg > 1:2818983/1‑50 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc > 1:279067/1‑50 (MQ=255) aaTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGc > 1:1785021/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc > 1:2294818/1‑50 (MQ=255) aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc < 1:1338837/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:198814/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:386947/50‑1 (MQ=255) ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc < 1:2905587/50‑1 (MQ=255) cACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCTGc > 1:2445757/1‑50 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt > 1:3576344/1‑49 (MQ=255) ccTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTc < 1:2631671/50‑1 (MQ=255) ttttttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCAt > 1:494989/1‑50 (MQ=255) ttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc > 1:1609797/1‑49 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa > 1:4216269/1‑50 (MQ=255) tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa > 1:711594/1‑50 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:1721233/1‑50 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:3725584/1‑50 (MQ=255) aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt > 1:107487/1‑50 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAtt < 1:2702670/50‑1 (MQ=255) ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAt < 1:2668689/49‑1 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa > 1:1240217/1‑50 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa < 1:3727110/50‑1 (MQ=255) cGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTa > 1:2927180/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3003211/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:4351019/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 1:3490437/50‑1 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:1012007/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3587329/1‑50 (MQ=255) gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc < 1:3548404/50‑1 (MQ=255) tttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:1535518/1‑49 (MQ=255) ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:3443909/1‑48 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:2025882/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc > 1:110251/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCa < 1:2946175/49‑1 (MQ=255) tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCCCATTGCCATTAc > 1:1336335/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAc > 1:2538176/1‑47 (MQ=255) tGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCa < 1:212009/49‑1 (MQ=255) gACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAg > 1:2597319/1‑49 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:1712740/50‑1 (MQ=255) cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt < 1:4059197/50‑1 (MQ=255) tGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc > 1:3125354/1‑50 (MQ=255) tGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc > 1:1306443/1‑50 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATc < 1:3025444/48‑1 (MQ=255) cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt < 1:1599045/49‑1 (MQ=255) cGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa > 1:4190252/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGaa < 1:1496325/49‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc < 1:1869480/50‑1 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:108186/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:1274407/1‑50 (MQ=255) gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc > 1:4182482/1‑50 (MQ=255) cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt < 1:1449345/50‑1 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:134432/1‑50 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg < 1:2636500/50‑1 (MQ=255) aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg > 1:2883342/1‑50 (MQ=255) gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg > 1:2737514/1‑50 (MQ=255) atcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt > 1:4209398/1‑50 (MQ=255) atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa > 1:894614/1‑50 (MQ=255) tGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:1832528/1‑49 (MQ=255) ggAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca > 1:2717783/1‑48 (MQ=255) | ACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA > NC_000913/3527714‑3527804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |