Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	379.2 / NA	79	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (38/41);  total (38/41)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234                                                  |                                              gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               <  1:4720611/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                              <  1:6701804/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                              <  1:6236048/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtt                                              <  1:5630727/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:890923/1‑49 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:460961/1‑49 (MQ=255)   cTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             <  1:41886/50‑2 (MQ=255)    ttttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAtta                                             >  1:5933249/1‑48 (MQ=255)      ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                            <  1:2240633/48‑3 (MQ=255)       tttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:6626772/1‑50 (MQ=39)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:7741081/1‑49 (MQ=38)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:1415399/1‑49 (MQ=38)        ttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGt                                        >  1:2297481/1‑49 (MQ=38)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:5102651/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      <  1:6996679/49‑1 (MQ=38)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      <  1:7887800/49‑1 (MQ=38)           tgCTTATCGCGATGATTTTCGCTGCGCTATCCGGGTAAA‑TTATAGTCATc                                    >  1:343608/1‑50 (MQ=38)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:1323982/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:2534396/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    >  1:678587/1‑50 (MQ=39)            gCTTATCGCGATGCTTTTCGCTGCGCTCTCAGGGTAAA‑TTATAGTCATCg                                   >  1:1998463/1‑50 (MQ=37)            gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   >  1:7437311/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  <  1:5268488/50‑1 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:1469444/1‑50 (MQ=39)             cTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   <  1:1955650/49‑1 (MQ=38)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:2185167/1‑49 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:7465572/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:5196887/48‑1 (MQ=38)                aTCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGt                                 <  1:5764270/48‑1 (MQ=38)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                              <  1:1775940/50‑1 (MQ=39)                 tCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTAtt                              <  1:7713818/50‑1 (MQ=39)                  cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1672278‑M1/1‑50 (MQ=255)                  cgcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:8282773‑M1/1‑50 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             <  1:8379467‑M1/49‑1 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             <  1:5075602‑M1/49‑1 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:1457128‑M1/1‑49 (MQ=255)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             <  1:7821366‑M1/49‑1 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:2548882‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:3138885‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:7085942‑M1/1‑48 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:3237506‑M1/1‑48 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:5470644‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:544961‑M1/50‑4 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          <  1:212790‑M1/50‑4 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:4477558‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         <  1:8224056‑M1/50‑5 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:4354925‑M1/1‑46 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:8117570‑M1/50‑6 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:1056498‑M1/50‑6 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagc                         >  1:6036969‑M1/1‑45 (MQ=255)                        gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       >  1:4491378‑M1/1‑44 (MQ=255)                        gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       >  1:5963811‑M1/1‑44 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:1888119‑M1/1‑43 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:3411019‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:879347‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:6563742‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       <  1:4448062‑M1/49‑7 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     <  1:6355479‑M1/50‑9 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:2377968‑M1/1‑42 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑GTATAGTCATCGGTATTAtagcaatc                     <  1:2839883‑M1/50‑9 (MQ=255)                            ttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatca                    <  1:8528141‑M1/49‑10 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  >  1:6031013‑M1/1‑39 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  >  1:5610687‑M1/1‑39 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaa                   <  1:4284964‑M1/49‑11 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:3965157‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:3192684‑M1/1‑38 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:5776798‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:3770112‑M1/49‑13 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:5321173‑M1/1‑36 (MQ=255)                                 tGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:4530551‑M1/1‑35 (MQ=255)                                  gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:1387874‑M1/1‑34 (MQ=255)                                  gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:3332382‑M1/1‑34 (MQ=255)                                  gcgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               >  1:6724591‑M1/1‑34 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccga              <  1:8457065‑M1/48‑16 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           <  1:5610326‑M1/50‑19 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:6412930‑M1/50‑20 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:7431767‑M1/50‑20 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcc          <  1:1527339‑M1/50‑20 (MQ=255)                                     cTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           >  1:237316‑M1/1‑31 (MQ=255)                                      tATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:3916456‑M1/1‑30 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:1402414‑M1/48‑21 (MQ=255)                                         cAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       >  1:4538331‑M1/1‑27 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     >  1:4386697‑M1/1‑26 (MQ=255)                                          aGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacata     <  1:4886289‑M1/50‑25 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    <  1:8079841‑M1/50‑26 (MQ=255)                                           gggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatat    >  1:622089‑M1/1‑25 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   <  1:6612840‑M1/50‑27 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  <  1:8078016‑M1/50‑28 (MQ=255)                                                  |                                              GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑