BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	477.8 / NA	120	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (65/55); total (65/55)

Only 100 of 120 total aligned reads displayed.

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:2208864/1‑50 (MQ=255)

gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:1262233/1‑50 (MQ=255)

gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                              >  1:5004113/1‑50 (MQ=255)

 cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             <  1:3682596/50‑1 (MQ=255)

 cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                             >  1:510187/1‑50 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:3642926/1‑50 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:4547721/1‑50 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            <  1:452562/50‑1 (MQ=255)

  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:6956286/1‑50 (MQ=255)

   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                            >  1:8168669/1‑49 (MQ=255)

   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                           <  1:5999798/50‑1 (MQ=255)

     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:2530836/50‑1 (MQ=255)

     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:5654950/50‑1 (MQ=255)

     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:7371968/50‑1 (MQ=255)

     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                         <  1:4639679/50‑1 (MQ=255)

         ttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:4522438/1‑49 (MQ=255)

           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:3771862/1‑47 (MQ=255)

           cGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                      >  1:8173128/1‑47 (MQ=255)

            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                  <  1:671773/50‑1 (MQ=255)

            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:6165873/1‑49 (MQ=255)

            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:6386920/1‑49 (MQ=255)

            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:1306533/1‑49 (MQ=255)

             ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                 <  1:6316238/50‑1 (MQ=255)

              cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                <  1:4609814/50‑1 (MQ=255)

              cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                <  1:4912207/50‑1 (MQ=255)

              cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                   >  1:3312519/1‑47 (MQ=255)

               tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                >  1:3862141/1‑49 (MQ=255)

               tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                >  1:1184294/1‑49 (MQ=255)

               tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                                >  1:188419/1‑49 (MQ=255)

                gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                              >  1:4996439/1‑50 (MQ=255)

                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaaga                               <  1:6900399/48‑1 (MQ=255)

                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                             >  1:5268127/1‑50 (MQ=255)

                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            >  1:5951826/1‑50 (MQ=255)

                  aCCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATc                            <  1:5947216/50‑1 (MQ=255)

                   ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                           >  1:5701781/1‑50 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:146529/1‑50 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          >  1:271290/1‑50 (MQ=255)

                    cTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATccc                          <  1:2630503/50‑1 (MQ=255)

                     ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         >  1:4023168/1‑50 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  1:3924298/48‑1 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                         <  1:5652114/48‑1 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                        <  1:4719607/49‑1 (MQ=255)

                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGc                       >  1:1619601/1‑50 (MQ=255)

                        ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                      >  1:1443908/1‑50 (MQ=255)

                         gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                     <  1:4937889/50‑1 (MQ=255)

                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    >  1:6593422/1‑50 (MQ=255)

                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    >  1:3491826/1‑50 (MQ=255)

                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                    >  1:7831324/1‑50 (MQ=255)

                           aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:5838514/1‑50 (MQ=255)

                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   <  1:2383674/49‑1 (MQ=255)

                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   >  1:4987788/1‑49 (MQ=255)

                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   <  1:208342/49‑1 (MQ=255)

                            tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                   <  1:8128282/49‑1 (MQ=255)

                             cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:7549751/1‑49 (MQ=255)

                             cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                  >  1:2995934/1‑49 (MQ=255)

                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaa                <  1:2496560/50‑1 (MQ=255)

                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 <  1:4480237/49‑1 (MQ=255)

                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:4391857/1‑49 (MQ=255)

                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:3222709/1‑49 (MQ=255)

                              aGATGCGGTAATCAGTGACATTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:2300591/1‑49 (MQ=38)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:4887559/50‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:7202031/50‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:1607032/50‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTaaa               <  1:7695863/50‑1 (MQ=255)

                               gATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:7555773/1‑48 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:6486284/1‑47 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:1545353/1‑47 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:6778651/1‑47 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:693365/1‑47 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                 >  1:2777539/1‑47 (MQ=255)

                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              <  1:29756/50‑1 (MQ=255)

                                 tGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaaga             <  1:4805175/50‑1 (MQ=255)

                                  gCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaag              >  1:6398651/1‑48 (MQ=255)

                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           <  1:7871354/50‑1 (MQ=255)

                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           >  1:7954189/1‑50 (MQ=255)

                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaag           <  1:76248/50‑1 (MQ=255)

                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:1073831/1‑50 (MQ=255)

                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:2127154/1‑49 (MQ=255)

                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:2476344/1‑49 (MQ=255)

                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc          >  1:6052440/1‑49 (MQ=255)

                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCg         <  1:2996452/50‑1 (MQ=255)

                                      tAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGa        >  1:1772851/1‑50 (MQ=255)

                                       aaTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       >  1:7196265/1‑50 (MQ=255)

                                       aaTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       >  1:1689230/1‑50 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:1389942/49‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:7855134/49‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt       <  1:779468/49‑1 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:8325401/1‑50 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:8270473/1‑50 (MQ=255)

                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      <  1:7770355/50‑1 (MQ=255)

                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg      >  1:4304601/1‑49 (MQ=255)

                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGc     <  1:2865147/50‑1 (MQ=255)

                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGc     <  1:841420/50‑1 (MQ=255)

                                         tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGc     <  1:4426753/50‑1 (MQ=255)

                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg   >  1:1280282/1‑50 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:285029/50‑1 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  >  1:424205/1‑50 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:2092380/50‑1 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:6674869/50‑1 (MQ=255)

                                            gTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAgc  <  1:1484267/50‑1 (MQ=255)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAGC  >  NC_000913/988452‑988545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`