Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,154,362 C→T A84V (GCC→GTC)  mltG → endolytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,154,3620CT100.0% 409.2 / NA 109A84V (GCC→GTC) mltGendolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (63/46);  total (63/46)

Only 100 of 109 total aligned reads displayed.

TTTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTG  >  NC_000913/1154315‑1154408
                                               |                                              
ttttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCg                                              >  1:5054661/1‑50 (MQ=255)
ttttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCg                                              >  1:7167629/1‑50 (MQ=255)
  ttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                             <  1:6915183/49‑1 (MQ=255)
  ttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                             <  1:3408967/49‑1 (MQ=255)
  ttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                             >  1:741114/1‑49 (MQ=255)
   tCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCggg                                            <  1:6991104/49‑1 (MQ=255)
   tCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           >  1:939602/1‑50 (MQ=255)
    cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           <  1:5643391/49‑1 (MQ=255)
    cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           <  1:3162311/49‑1 (MQ=255)
    cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           <  1:2370238/49‑1 (MQ=255)
    cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                           <  1:8040296/49‑1 (MQ=255)
    cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGAc                                          >  1:1386349/1‑50 (MQ=255)
     aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGAc                                          <  1:3968300/49‑1 (MQ=255)
     aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                         >  1:1877649/1‑50 (MQ=255)
      aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACtt                                        <  1:5853720/50‑1 (MQ=255)
      aTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                         >  1:196217/1‑49 (MQ=255)
       tGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                       >  1:439028/1‑50 (MQ=255)
       tGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTa                                       >  1:4378318/1‑50 (MQ=255)
        ggCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAc                                      >  1:3238005/1‑50 (MQ=255)
         gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                     <  1:8360585/50‑1 (MQ=255)
          ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                    >  1:4599374/1‑50 (MQ=255)
          ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                    >  1:563085/1‑50 (MQ=255)
          ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                    <  1:3992530/50‑1 (MQ=255)
          ctgctgCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCg                                    <  1:5709982/50‑1 (MQ=255)
           tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                   >  1:903273/1‑50 (MQ=255)
           tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                   >  1:4859520/1‑50 (MQ=255)
           tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                   >  1:2837970/1‑50 (MQ=255)
           tgctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                   >  1:2359469/1‑50 (MQ=255)
            gctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCt                                  >  1:1104119/1‑50 (MQ=255)
            gctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCt                                  >  1:4862952/1‑50 (MQ=255)
             ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGc                                   <  1:1364273/48‑1 (MQ=255)
             ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 <  1:5794564/50‑1 (MQ=255)
             ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 >  1:7479395/1‑50 (MQ=255)
             ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 <  1:3419829/50‑1 (MQ=255)
              tgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                                 >  1:8562697/1‑49 (MQ=255)
                cGTATCGAAGCGGATCTTTCTCACTTTAAAGTCGGGACTTGCCGCTTTa                               <  1:353188/49‑1 (MQ=38)
                cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCttt                                <  1:3932351/48‑1 (MQ=255)
                cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTac                              <  1:5277991/50‑1 (MQ=255)
                cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                               <  1:489103/49‑1 (MQ=255)
                cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTa                               >  1:2175961/1‑49 (MQ=255)
                  tATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTacac                            <  1:6050812/50‑1 (MQ=255)
                  tATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTacac                            >  1:549239/1‑50 (MQ=255)
                   aTCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACAcc                           >  1:8406916/1‑50 (MQ=255)
                    tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:1396889/1‑50 (MQ=255)
                    tCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:6600796/1‑50 (MQ=255)
                      gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:748646/1‑48 (MQ=255)
                      gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:8612199/1‑48 (MQ=255)
                      gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:7448806/1‑48 (MQ=255)
                      gAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGc                         <  1:3510870/49‑1 (MQ=255)
                       aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:876832/1‑47 (MQ=255)
                       aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:3074800/1‑47 (MQ=255)
                        aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:4903323/1‑46 (MQ=255)
                        aCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCg                          >  1:2040738/1‑46 (MQ=255)
                         ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                     >  1:2038413/1‑50 (MQ=255)
                          cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACGCCGCAGATg                    <  1:8375068/50‑1 (MQ=255)
                          cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                     >  1:6220394/1‑49 (MQ=255)
                          cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATg                    <  1:5263169/50‑1 (MQ=255)
                           ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                   <  1:8574212/50‑1 (MQ=255)
                           ggATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGa                   >  1:851604/1‑50 (MQ=255)
                            gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATggc                  <  1:3983609/50‑3 (MQ=255)
                            gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAc                  >  1:8298856/1‑50 (MQ=255)
                            gATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAc                  >  1:220098/1‑50 (MQ=255)
                             aTCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                 >  1:3610653/1‑50 (MQ=255)
                             aTCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                 >  1:5496665/1‑50 (MQ=255)
                              tCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCg                >  1:6205601/1‑50 (MQ=255)
                              tCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCg                >  1:2295395/1‑50 (MQ=255)
                               cTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt               >  1:7547243/1‑50 (MQ=255)
                                attCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg              >  1:5658203/2‑50 (MQ=255)
                                 ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc             >  1:419176/1‑50 (MQ=255)
                                 ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc             >  1:2291232/1‑50 (MQ=255)
                                 ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc             >  1:6395244/1‑50 (MQ=255)
                                 ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc             >  1:6508967/1‑50 (MQ=255)
                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc                 <  1:5547654/45‑1 (MQ=255)
                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCg                <  1:2329609/46‑1 (MQ=255)
                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt               <  1:3807305/47‑1 (MQ=255)
                                  tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGt               <  1:7652093/47‑1 (MQ=255)
                                   ctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg            <  1:5691386/49‑1 (MQ=255)
                                    tcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGcgcg          >  1:5281664/1‑50 (MQ=255)
                                     cACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:3968672/1‑48 (MQ=255)
                                      aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           <  1:3436186/47‑1 (MQ=255)
                                      aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           >  1:4417108/1‑47 (MQ=255)
                                      aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGcgcg          <  1:6934503/48‑1 (MQ=255)
                                      aCTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCga         <  1:2660883/49‑1 (MQ=255)
                                      aCTTTAAAGTAGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc           <  1:5796782/47‑1 (MQ=39)
                                       cTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCgaga       <  1:3503870/50‑1 (MQ=255)
                                         ttAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg     >  1:2091603/1‑50 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt      <  1:5235143/48‑1 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt      <  1:7729547/48‑1 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg     <  1:2750610/49‑1 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg     <  1:31594/49‑1 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg     <  1:2759829/49‑1 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATg     <  1:1153048/49‑1 (MQ=255)
                                          tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc    >  1:1869797/1‑50 (MQ=255)
                                           aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc    <  1:6812255/49‑1 (MQ=255)
                                           aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc    <  1:1165036/49‑1 (MQ=255)
                                           aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt   >  1:7917526/1‑50 (MQ=255)
                                           aaaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCt   >  1:4144824/1‑50 (MQ=255)
                                            aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTg  <  1:6744799/50‑1 (MQ=255)
                                            aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTg  <  1:6310960/50‑1 (MQ=255)
                                            aaGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTg  <  1:4897867/50‑1 (MQ=255)
                                               |                                              
TTTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGCTG  >  NC_000913/1154315‑1154408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑