| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,270,811 | C→T | V347M (GTG→ATG) | chaA ← | Na(+)/K(+):H(+) antiporter ChaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,270,811 | 0 | C | T | 99.0% | 370.4 / ‑6.0 | 96 | V347M (GTG→ATG) | chaA | Na(+)/K(+):H(+) antiporter ChaA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (52/43); minor base A (0/1); total (52/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG > NC_000913/1270764‑1270857 | catcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:6725211/1‑50 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:6610082/1‑49 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:4134716/1‑49 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt < 1:8220475/49‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt < 1:4430624/49‑1 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt > 1:2094273/1‑48 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa < 1:200248/50‑1 (MQ=255) tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTa < 1:3538306/50‑1 (MQ=255) caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:6648465/1‑50 (MQ=255) caAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:7432068/1‑50 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg > 1:5065427/1‑49 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg < 1:2710094/49‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAg < 1:76483/49‑1 (MQ=255) aaaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGt < 1:5130739/50‑1 (MQ=255) aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa < 1:356625/50‑1 (MQ=255) aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa > 1:2016490/1‑50 (MQ=255) aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa > 1:2788625/1‑50 (MQ=255) aTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTAc < 1:4124557/50‑1 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:5064123/50‑1 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg > 1:840718/1‑50 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg > 1:5880622/1‑50 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:2339154/50‑1 (MQ=255) tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg < 1:7807680/50‑1 (MQ=255) aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt > 1:7404381/1‑50 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt < 1:3686104/49‑1 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGt < 1:4039682/49‑1 (MQ=255) ggcggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTc < 1:6269848/50‑1 (MQ=255) ggcggcAAACAGTGCCAGATGCGCAGCGCCATTGAGCATGTTAGTACGTc < 1:2267847/50‑1 (MQ=38) cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg > 1:2692667/1‑50 (MQ=255) cggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCg > 1:2630025/1‑50 (MQ=255) ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg > 1:626356/1‑50 (MQ=255) ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg > 1:8348103/1‑50 (MQ=255) ggcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCgg > 1:1389014/1‑50 (MQ=255) gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt < 1:7878798/50‑1 (MQ=255) gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt > 1:6239641/1‑50 (MQ=255) cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:6636479/1‑50 (MQ=255) cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:6540345/1‑50 (MQ=255) cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:5571857/1‑50 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCCTTGAGCATGTTAGTACGTCCGGTg > 1:5322276/1‑49 (MQ=39) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTgg < 1:5786354/50‑1 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:4251344/1‑49 (MQ=255) aaaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:5054100/1‑49 (MQ=255) aaCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGga < 1:5116369/50‑1 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:3756089/1‑47 (MQ=255) aCAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg > 1:4905032/1‑47 (MQ=255) cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgaga < 1:3806313/50‑1 (MQ=255) cAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGgag > 1:6729738/1‑49 (MQ=255) aGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGaa < 1:7226948/50‑1 (MQ=255) gTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg < 1:4732531/50‑1 (MQ=255) tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg < 1:1149128/50‑1 (MQ=255) tGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAgg < 1:5901308/50‑1 (MQ=255) gCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGGGAAg > 1:1695026/1‑48 (MQ=39) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:560416/1‑47 (MQ=255) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:5558725/1‑47 (MQ=255) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag < 1:8138588/50‑1 (MQ=255) ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgag < 1:3810492/50‑1 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:8267932/1‑46 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAg > 1:8444456/1‑46 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga < 1:550521/50‑1 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga < 1:2440683/50‑1 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga < 1:6618200/50‑1 (MQ=255) cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga < 1:8146426/50‑1 (MQ=255) aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga > 1:3235324/1‑49 (MQ=255) gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata > 1:6222837/1‑50 (MQ=255) gATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGata < 1:3713591/50‑1 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat < 1:6645090/50‑1 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat < 1:5685814/50‑1 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat > 1:2574442/1‑50 (MQ=255) aTGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGatat < 1:2398368/50‑1 (MQ=255) tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATg > 1:7016143/1‑50 (MQ=255) tgcgctgcgcCATTGAGCATGTTAGTACGTCCGGCGGAGAAGGAGATATg > 1:2961746/1‑50 (MQ=255) gcgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg > 1:2695426/1‑50 (MQ=255) cgctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATgg > 1:2991773/1‑49 (MQ=255) gctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:4666934/1‑50 (MQ=255) ctgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcac < 1:5144845/50‑1 (MQ=255) tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:1702335/1‑48 (MQ=255) tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:6602883/1‑48 (MQ=255) tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca > 1:4667729/1‑48 (MQ=255) gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGCGATATGGCACAg > 1:8617267/1‑50 (MQ=255) gcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:2276112/1‑50 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:3281085/48‑1 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:521186/1‑49 (MQ=255) cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAg > 1:4912346/1‑49 (MQ=255) cgcCATTGAACAAGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca < 1:4500279/48‑1 (MQ=39) gcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCa > 1:6156901/1‑50 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACt > 1:510328/1‑49 (MQ=255) aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa < 1:4542165/50‑1 (MQ=255) ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa < 1:4468700/50‑1 (MQ=255) ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaa < 1:3093786/50‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa < 1:9101/49‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa < 1:6879288/49‑1 (MQ=255) gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa < 1:4745506/49‑1 (MQ=255) aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa > 1:2926046/1‑48 (MQ=255) aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa > 1:6091680/1‑48 (MQ=255) aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAag < 1:3634551/49‑1 (MQ=255) gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag > 1:5319958/1‑50 (MQ=255) | CATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG > NC_000913/1270764‑1270857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |