BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	398.7 / NA	102	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/49); total (53/49)

Only 100 of 108 total aligned reads displayed.

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074

aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                              <  1:5477200/50‑1 (MQ=255)

 aTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctc                                             <  1:6705334/50‑1 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:3737305/50‑1 (MQ=255)

  tAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            <  1:67182/50‑1 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:3626424/1‑48 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                            >  1:7144482/1‑48 (MQ=255)

    gTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCa                                          <  1:4170111/50‑1 (MQ=255)

     tCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  1:1552691/1‑50 (MQ=255)

      ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                         >  1:8458096/1‑49 (MQ=255)

       cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                       >  1:337460/1‑50 (MQ=255)

        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:8488056/1‑50 (MQ=255)

        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:3979239/1‑50 (MQ=255)

        aGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:8243153/1‑50 (MQ=255)

         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:3429516/1‑49 (MQ=255)

         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:5507132/1‑49 (MQ=255)

         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:4623032/1‑49 (MQ=255)

         gTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATc                                     <  1:1468611/50‑1 (MQ=255)

          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:5241413/1‑48 (MQ=255)

          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:3790658/1‑48 (MQ=255)

          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:7658509/1‑48 (MQ=255)

          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                      >  1:6772839/1‑48 (MQ=255)

          tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCa                                    <  1:1722349/50‑1 (MQ=255)

           aTGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                   >  1:2425645/1‑50 (MQ=255)

            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  >  1:2217770/1‑50 (MQ=255)

            tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                  >  1:1261843/1‑50 (MQ=255)

             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:3295007/1‑50 (MQ=255)

             gTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 >  1:3431044/1‑50 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                                >  1:6203805/1‑50 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 <  1:5518145/49‑1 (MQ=255)

              ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAggg                                 <  1:6436622/49‑1 (MQ=255)

               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               >  1:5505440/1‑50 (MQ=255)

               tCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCACTATCAGGGgc                               >  1:2808891/1‑50 (MQ=255)

                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               <  1:5307214/49‑1 (MQ=255)

                ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                               <  1:1005656/49‑1 (MQ=255)

                 cTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             <  1:1842969/50‑1 (MQ=255)

                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:8453246/1‑49 (MQ=255)

                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:4293149/1‑49 (MQ=255)

                  tttCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcgc                             >  1:4622676/1‑49 (MQ=255)

                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGcgcg                            <  1:2085180/48‑1 (MQ=255)

                    tCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                          >  1:4148319/1‑50 (MQ=255)

                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:1610751/1‑50 (MQ=255)

                     cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         >  1:2465356/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:2720277/49‑1 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:5990077/49‑1 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                         <  1:5005436/49‑1 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  1:5746148/1‑50 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        <  1:6900611/50‑1 (MQ=255)

                      gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACg                        >  1:2768510/1‑50 (MQ=255)

                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCCGGGGCGCGAACGc                       >  1:4700632/1‑50 (MQ=255)

                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       >  1:240664/1‑50 (MQ=255)

                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       <  1:7878768/50‑1 (MQ=255)

                       aTAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGc                       <  1:5879236/50‑1 (MQ=255)

                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  1:1302547/50‑1 (MQ=255)

                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  1:3512415/50‑1 (MQ=255)

                        tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                      <  1:5962837/50‑1 (MQ=255)

                         aaaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:7999843/1‑50 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  1:2977609/49‑1 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  1:2344752/49‑1 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     >  1:6975781/1‑49 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                     <  1:3704961/49‑1 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:6526561/50‑1 (MQ=255)

                          aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    <  1:6388731/50‑1 (MQ=255)

                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGc                    >  1:4846191/1‑49 (MQ=255)

                           aCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCa                   <  1:8124404/50‑1 (MQ=255)

                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  <  1:6125660/50‑1 (MQ=255)

                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  <  1:6704112/50‑1 (MQ=255)

                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:7785674/1‑50 (MQ=255)

                            cGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCaa                  >  1:3548993/1‑50 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:5612376/1‑50 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:5392449/1‑50 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 <  1:4725791/50‑1 (MQ=255)

                             gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt                 >  1:8088228/1‑50 (MQ=255)

                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  1:5898491/50‑1 (MQ=255)

                              tGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATg                <  1:3337003/50‑1 (MQ=255)

                               gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               >  1:7230647/1‑50 (MQ=255)

                               gcatggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGa               >  1:369952/1‑50 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:1758319/1‑50 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  1:6908162/50‑1 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             >  1:526937/1‑50 (MQ=255)

                                 atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt             <  1:3274518/50‑1 (MQ=255)

                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            <  1:1688861/50‑1 (MQ=255)

                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:825768/1‑50 (MQ=255)

                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            >  1:4931790/1‑50 (MQ=255)

                                  tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt            <  1:7748732/50‑1 (MQ=255)

                                    gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt           <  1:7055971/49‑1 (MQ=255)

                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:3457928/1‑50 (MQ=255)

                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  1:2036926/50‑1 (MQ=255)

                                     catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         <  1:5154142/50‑1 (MQ=255)

                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc         >  1:7150757/1‑49 (MQ=255)

                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt        <  1:7039856/50‑1 (MQ=255)

                                      atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt        <  1:2758199/50‑1 (MQ=255)

                                       tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa       <  1:1135965/50‑1 (MQ=255)

                                       tgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTa       <  1:4166826/50‑1 (MQ=255)

                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  1:501343/1‑50 (MQ=255)

                                        gCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      >  1:1189865/1‑50 (MQ=255)

                                         cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAt      <  1:4362111/49‑1 (MQ=255)

                                          gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg    <  1:7135502/50‑1 (MQ=255)

                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:1003272/50‑1 (MQ=255)

                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:4559823/50‑1 (MQ=255)

                                            gAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTggg  <  1:4964272/50‑1 (MQ=255)

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGG  >  NC_000913/118981‑119074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`