BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	418.6 / NA	107	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (47/60); total (47/60)

Only 100 of 107 total aligned reads displayed.

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382508‑1382600

ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             >  1:6731775/1‑50 (MQ=255)

ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             >  1:365275/1‑50 (MQ=255)

ttGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             >  1:1077198/1‑50 (MQ=255)

  gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:2506834/48‑1 (MQ=255)

  gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:2368715/48‑1 (MQ=255)

  gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:5970457/48‑1 (MQ=255)

  gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCg                                             <  1:3392257/48‑1 (MQ=255)

  gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                            >  1:4110316/1‑49 (MQ=255)

  gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                            <  1:4287170/49‑1 (MQ=255)

    gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                          >  1:5877866/1‑49 (MQ=255)

    gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                          >  1:812595/1‑49 (MQ=255)

    gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                          >  1:8566092/1‑49 (MQ=255)

    gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         <  1:2672455/50‑1 (MQ=255)

    gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         <  1:567481/50‑1 (MQ=255)

     aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                         >  1:7340449/1‑49 (MQ=255)

     aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                        <  1:5147110/50‑1 (MQ=255)

     aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                        <  1:4313656/50‑1 (MQ=255)

     aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                        <  1:2070511/50‑1 (MQ=255)

     aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                        <  1:1912829/50‑1 (MQ=255)

      gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                       <  1:5652053/50‑1 (MQ=255)

       tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                      <  1:7751591/50‑1 (MQ=255)

       tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                      <  1:3009032/50‑1 (MQ=255)

       tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                      >  1:286044/1‑50 (MQ=255)

         aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                    >  1:5233271/1‑50 (MQ=255)

         aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                    >  1:5563923/1‑50 (MQ=255)

         aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                    >  1:6630984/1‑50 (MQ=255)

         aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACgg                                    >  1:8575253/1‑50 (MQ=255)

         aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                     <  1:3024421/49‑1 (MQ=255)

          aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:7667532/1‑50 (MQ=255)

          aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:6111748/1‑50 (MQ=255)

          aCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   <  1:3038493/50‑1 (MQ=255)

           cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                  <  1:5939209/50‑1 (MQ=255)

           cTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTg                                  <  1:6471943/50‑1 (MQ=255)

            tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                   >  1:4951501/1‑48 (MQ=255)

             attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                >  1:5908650/1‑50 (MQ=255)

             attatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                >  1:2143765/1‑50 (MQ=255)

              ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGa                                 <  1:6097700/48‑1 (MQ=255)

              ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                <  1:1237001/49‑1 (MQ=255)

              ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                <  1:8564166/49‑1 (MQ=255)

              ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                               >  1:6382518/1‑50 (MQ=255)

              ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGt                               <  1:1808116/50‑1 (MQ=255)

              ttatCTCGAACGCGGCTTCAATATAGACGACAGTCGCAATAACGGTGa                                 <  1:3947998/48‑1 (MQ=255)

               tatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                              >  1:8244068/1‑50 (MQ=255)

                atCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtt                              <  1:2863551/49‑1 (MQ=255)

                 tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                            >  1:758691/1‑50 (MQ=255)

                 tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                             <  1:8409/49‑1 (MQ=255)

                  ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                           >  1:6689860/1‑50 (MQ=255)

                   tcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                           >  1:6610803/1‑49 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGCGTTTTCCAc                        >  1:6334806/1‑50 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGtttt                            <  1:5837932/46‑1 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                         <  1:5048632/49‑1 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                         <  1:8573068/49‑1 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                        <  1:7378048/50‑1 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                        >  1:4852022/1‑50 (MQ=255)

                     gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                        >  1:3806304/1‑50 (MQ=255)

                      aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                       <  1:1179247/50‑1 (MQ=255)

                      aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                       >  1:413078/1‑50 (MQ=255)

                      aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                       <  1:1652780/50‑1 (MQ=255)

                      aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                       >  1:3261360/1‑50 (MQ=255)

                          cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGc                      >  1:5600641/1‑47 (MQ=255)

                          cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   <  1:1904057/50‑1 (MQ=255)

                          cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   <  1:2363956/50‑1 (MQ=255)

                          cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   <  1:2365669/50‑1 (MQ=255)

                           ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   >  1:2865175/1‑49 (MQ=255)

                           ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   >  1:2414493/1‑49 (MQ=255)

                           ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   >  1:4160691/1‑49 (MQ=255)

                           gccTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                   >  1:8519958/3‑49 (MQ=255)

                            gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaa                 <  1:6995825/50‑1 (MQ=255)

                            gCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaa                 <  1:2887044/50‑1 (MQ=255)

                             cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                <  1:2699870/50‑1 (MQ=255)

                             cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                >  1:827082/1‑50 (MQ=255)

                             cTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGaaa                <  1:2873109/50‑1 (MQ=255)

                              ttCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt               >  1:8460467/1‑50 (MQ=255)

                                cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt               <  1:126873/48‑1 (MQ=255)

                                cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc             >  1:1753576/1‑50 (MQ=255)

                                cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc             >  1:7191165/1‑50 (MQ=255)

                                cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc             >  1:6815925/1‑50 (MQ=255)

                                 aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg            <  1:1186887/50‑1 (MQ=255)

                                  atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc           >  1:2768350/1‑50 (MQ=255)

                                  atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc           <  1:1487673/50‑1 (MQ=255)

                                  atatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgc           >  1:2917516/1‑50 (MQ=255)

                                   tatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg          <  1:2067482/50‑1 (MQ=255)

                                   tatGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcgcg          <  1:3731626/50‑1 (MQ=255)

                                    atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         >  1:4653713/1‑50 (MQ=255)

                                    atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         >  1:678667/1‑50 (MQ=255)

                                    atGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         >  1:2613300/1‑50 (MQ=255)

                                     tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCgcgc         <  1:605121/49‑1 (MQ=255)

                                     tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        <  1:6937000/50‑1 (MQ=255)

                                     tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        <  1:5268582/50‑1 (MQ=255)

                                     tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        <  1:4498026/50‑1 (MQ=255)

                                     tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        <  1:2246269/50‑1 (MQ=255)

                                      ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc        >  1:7413907/1‑49 (MQ=255)

                                       gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt       <  1:8432038/49‑1 (MQ=255)

                                       gacgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCt       <  1:2060768/49‑1 (MQ=255)

                                        acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt     <  1:171767/50‑1 (MQ=255)

                                         cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt    <  1:5038236/50‑1 (MQ=255)

                                          gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt   >  1:6959978/1‑50 (MQ=255)

                                          gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt   <  1:1831165/50‑1 (MQ=255)

                                          gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt   <  1:1162330/50‑1 (MQ=255)

                                           acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg  <  1:1939619/50‑1 (MQ=255)

TTGCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTG  >  NC_000913/1382508‑1382600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`