BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,562,085	C→T	A133T (GCA→ACA)	ddpA ←	putative D,D‑dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,562,085	0	C	T	100.0%	458.9 / NA	117	A133T (GCA→ACA)	ddpA	putative D,D‑dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (54/63); total (54/63)

Only 100 of 119 total aligned reads displayed.

ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGT  >  NC_000913/1562039‑1562131

aCCGTATGTTCGTCGGGGGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             <  1:4037098/50‑1 (MQ=255)

aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGttt                                              <  1:6139462/49‑1 (MQ=255)

aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             <  1:3196677/50‑1 (MQ=255)

aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             >  1:789672/1‑50 (MQ=255)

aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             >  1:224851/1‑50 (MQ=255)

aCCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTc                                             <  1:4545817/50‑1 (MQ=255)

 ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                            <  1:7696655/50‑1 (MQ=255)

 ccGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTct                                            <  1:7402283/50‑1 (MQ=255)

  cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctg                                           <  1:5221100/50‑1 (MQ=255)

  cGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctg                                           >  1:5749243/1‑50 (MQ=255)

      tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTctgctg                                        <  1:7753194/49‑1 (MQ=255)

      tGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTgg                                       <  1:4206950/50‑1 (MQ=255)

        ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcc                                     >  1:900950/1‑50 (MQ=255)

        ttCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGc                                      <  1:4404655/49‑1 (MQ=255)

         tcgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGccc                                    >  1:4289146/1‑50 (MQ=255)

          cgtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc                                   >  1:759314/1‑50 (MQ=255)

           gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGtccc                                   <  1:5082238/49‑1 (MQ=255)

           gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGcccc                                   <  1:1327460/49‑1 (MQ=255)

           gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  <  1:592690/50‑1 (MQ=255)

           gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  >  1:1521577/1‑50 (MQ=255)

           gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  >  1:7125505/1‑50 (MQ=255)

           gtcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCt                                  >  1:3477659/1‑50 (MQ=255)

            tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTg                                 >  1:3753576/1‑50 (MQ=255)

            tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTg                                 >  1:3213024/1‑50 (MQ=255)

            tcgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTg                                 <  1:2906332/50‑1 (MQ=255)

             cgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTgg                                >  1:654598/1‑50 (MQ=255)

             cgGGAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTgg                                >  1:7354464/1‑50 (MQ=255)

              gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGc                               <  1:1082310/50‑1 (MQ=255)

              gggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGc                               <  1:2213785/50‑1 (MQ=255)

               ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              <  1:8216624/50‑1 (MQ=255)

               ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              <  1:8134389/50‑1 (MQ=255)

               ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              <  1:1218370/50‑1 (MQ=255)

               ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              <  1:7586475/50‑1 (MQ=255)

               ggAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGcc                              >  1:1594382/1‑50 (MQ=255)

                gAGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCg                             <  1:5057692/50‑1 (MQ=255)

                 aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            <  1:2965903/50‑1 (MQ=255)

                 aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            >  1:761554/1‑50 (MQ=255)

                 aGCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGa                            >  1:4799061/1‑50 (MQ=255)

                  gCATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAt                           <  1:1621126/50‑1 (MQ=255)

                   cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                          >  1:1043030/1‑50 (MQ=255)

                   cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                          >  1:7503179/1‑50 (MQ=255)

                   cATCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtt                          >  1:6012575/1‑50 (MQ=255)

                    aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttt                         <  1:8456022/50‑1 (MQ=255)

                    aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttt                         >  1:7108528/1‑50 (MQ=255)

                    aTCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttt                         >  1:5260649/1‑50 (MQ=255)

                     tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  1:2675491/1‑50 (MQ=255)

                     tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  1:766949/1‑50 (MQ=255)

                     tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  1:5361798/1‑50 (MQ=255)

                     tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  1:5230301/1‑50 (MQ=255)

                     tCAATCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        <  1:1339099/50‑1 (MQ=255)

                       aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttttt                       <  1:3939483/49‑1 (MQ=255)

                       aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAttttt                       <  1:1463728/49‑1 (MQ=255)

                       aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGAtttt                        >  1:5911159/1‑48 (MQ=255)

                       aaTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTta                      <  1:6428884/50‑1 (MQ=255)

                        aTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                     <  1:2406835/50‑1 (MQ=255)

                        aTCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                     <  1:7098215/50‑1 (MQ=255)

                         tCTTTAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtag                     >  1:1984022/1‑49 (MQ=255)

                           tttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  >  1:1842343/1‑50 (MQ=255)

                            ttAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGc                 >  1:8622029/1‑50 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  <  1:7551889/48‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  <  1:1271721/48‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagtag                  <  1:7954578/48‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagta                   <  1:2672718/47‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagta                   <  1:2252182/47‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagta                   <  1:3890048/47‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTtagta                   <  1:177740/47‑1 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc                >  1:6673609/1‑50 (MQ=255)

                             tAAATCTTTGGGAAATGTTTCTGCTGGCACCTGGCCGATTTTTAGTAGcc                >  1:7984201/1‑50 (MQ=255)

                              aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGcc                <  1:7095517/49‑1 (MQ=255)

                              aaaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg               >  1:2534510/1‑50 (MQ=255)

                               aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCg               <  1:2665219/49‑1 (MQ=255)

                               aaTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGc              <  1:3060237/50‑1 (MQ=255)

                                aTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCt             <  1:2323025/50‑1 (MQ=255)

                                aTCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCt             <  1:8524395/50‑1 (MQ=255)

                                 tCTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCt             >  1:2207967/1‑49 (MQ=255)

                                  cTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc            >  1:8177548/1‑49 (MQ=255)

                                  cTTTGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc            >  1:2202820/1‑49 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTc            <  1:722626/46‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaaa         <  1:3988838/49‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          >  1:3367761/1‑48 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          <  1:4358104/48‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          <  1:4427991/48‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          <  1:1083149/48‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa           <  1:6795567/47‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa           <  1:1250520/47‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCa           <  1:7773207/47‑1 (MQ=255)

                                     tGGGAAATGTTTCTGCTGGCACCTGGCCGATTTTTAGTAGCCGCTc            <  1:6517590/46‑1 (MQ=255)

                                      gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          >  1:5039559/1‑47 (MQ=255)

                                      gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          >  1:1368724/1‑47 (MQ=255)

                                      gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          >  1:6093954/1‑47 (MQ=255)

                                      gggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCaa          >  1:7044197/1‑47 (MQ=255)

                                       ggAAATGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      <  1:8073787/50‑1 (MQ=255)

                                         aaaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      >  1:4152283/1‑48 (MQ=255)

                                         aaaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaaga      >  1:7289399/1‑48 (MQ=255)

                                          aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag   >  1:4426236/1‑50 (MQ=255)

                                          aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag   >  1:807221/1‑50 (MQ=255)

                                          aaTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAaaagaaag   >  1:8263133/1‑50 (MQ=255)

                                           aTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGt  >  1:2996840/1‑50 (MQ=255)

                                           aTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGt  <  1:5140158/50‑1 (MQ=255)

                                           aTGTTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGt  >  1:2897429/1‑50 (MQ=255)

ACCGTATGTTCGTCGGGAGCATCAATCTTTAAATCTTTGGGAAATGCTTCTGCTGGCCCCTGGCCGATTTTTAGTAGCCGCTCAAAAGAAAGT  >  NC_000913/1562039‑1562131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`