Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	365.5 / NA	90	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (43/47);  total (43/47)

GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801                                                |                                               gTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGa                                              <  1:2423148/50‑1 (MQ=255)  tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             <  1:4991532/50‑1 (MQ=255)  tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             >  1:1662910/1‑50 (MQ=255)  tGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGaa                                             <  1:4205702/50‑1 (MQ=255)   gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:5173995/1‑50 (MQ=255)   gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:2915943/1‑50 (MQ=255)   gcgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:441873/1‑50 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           <  1:518579/50‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  1:8541673/49‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            <  1:5601644/49‑1 (MQ=255)    cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAt                                            >  1:3180893/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                           >  1:3977206/1‑49 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:3528491/50‑1 (MQ=255)     gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                          <  1:2835929/50‑1 (MQ=255)      ccAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaa                                         <  1:3518149/50‑1 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:4039094/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:1578934/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:8023989/1‑50 (MQ=255)       cAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTaaa                                        >  1:6833664/1‑50 (MQ=255)        aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                       >  1:3463851/1‑50 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      <  1:1051891/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      <  1:1483648/50‑1 (MQ=255)         aGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      <  1:7912755/50‑1 (MQ=255)          gTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATg                                      >  1:5629393/1‑49 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    <  1:3668073/50‑1 (MQ=255)           ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                    >  1:3494801/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:7390896/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:1247747/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                   <  1:5652990/49‑1 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:5153809/1‑50 (MQ=255)             ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  >  1:5965041/1‑50 (MQ=255)              tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                  <  1:7222372/49‑1 (MQ=255)               ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                 >  1:7983529/1‑49 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:3696651/49‑1 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:4536503/49‑1 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                >  1:8448892/1‑49 (MQ=255)                tcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCa                                <  1:3105646/49‑1 (MQ=255)                 cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               <  1:5153017/49‑1 (MQ=255)                 cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               <  1:3865203/49‑1 (MQ=255)                 cctcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                               >  1:1678441/3‑49 (MQ=255)                   tcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            >  1:8245511/1‑50 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  1:1701518/49‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaaaa                            <  1:5663477/49‑1 (MQ=255)                    cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                              <  1:4499517/47‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcg                          >  1:6366621/1‑50 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:866335/49‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:1480853/49‑1 (MQ=255)                     ccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAc                           <  1:3161589/49‑1 (MQ=255)                      cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                         >  1:3903251/1‑50 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        <  1:3533257/50‑1 (MQ=255)                       aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                        <  1:2280763/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                      <  1:8351293/50‑1 (MQ=255)                         gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                      >  1:2350627/1‑50 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:960468/50‑1 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     >  1:3976779/1‑50 (MQ=255)                          agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                     <  1:3232975/50‑1 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:892746/1‑50 (MQ=255)                           gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                    >  1:4725631/1‑50 (MQ=255)                            tCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGc                   <  1:5630651/50‑1 (MQ=255)                             cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  >  1:2686113/1‑50 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                  <  1:56355/49‑1 (MQ=255)                              gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                 >  1:7848922/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              >  1:5228396/1‑50 (MQ=255)                                 cGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGAc              <  1:5983809/50‑1 (MQ=255)                                  gTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACt             <  1:2405946/50‑1 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            >  1:7290460/1‑50 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  1:337579/50‑1 (MQ=255)                                   ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt            <  1:3574182/50‑1 (MQ=255)                                    tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           >  1:2208309/1‑50 (MQ=255)                                     ccAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc           <  1:5646263/49‑1 (MQ=255)                                      cAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGc         <  1:7410202/50‑1 (MQ=255)                                       aGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:8101905/1‑50 (MQ=255)                                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:8567737/1‑49 (MQ=255)                                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGcc        >  1:1821192/1‑49 (MQ=255)                                        gtgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCg       <  1:6650827/50‑1 (MQ=255)                                         tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa      >  1:6918081/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  1:2745138/49‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc     <  1:5565399/49‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:1140139/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:3236406/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    >  1:1258729/1‑50 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:1449564/50‑1 (MQ=255)                                           tttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACg    <  1:2684239/50‑1 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:8264549/1‑50 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:1358406/1‑50 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:1146031/1‑50 (MQ=255)                                            ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   >  1:2770225/1‑50 (MQ=255)                                             tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg   <  1:5604837/49‑1 (MQ=255)                                             tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  1:1794775/1‑50 (MQ=255)                                             tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGt  >  1:8076319/1‑50 (MQ=255)                                                |                                               GTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGGT  >  NC_000913/1742708‑1742801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑