BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	468.2 / NA	118	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (47/71); total (47/71)

Only 100 of 118 total aligned reads displayed.

CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTG  >  NC_000913/1811162‑1811254

cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTg                                              <  1:6478855/49‑1 (MQ=255)

cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGc                                             <  1:4104642/50‑1 (MQ=255)

 aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                            >  1:5098076/1‑50 (MQ=255)

 aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGAGCGGCTGcc                                            >  1:8073288/1‑50 (MQ=39)

  aaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGccc                                           >  1:8275117/1‑50 (MQ=255)

     gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                        >  1:8507447/1‑50 (MQ=255)

     gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                        >  1:8176170/1‑50 (MQ=255)

      aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                        <  1:4013010/49‑1 (MQ=255)

      aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       <  1:2361608/50‑1 (MQ=255)

      aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       <  1:2312828/50‑1 (MQ=255)

      aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       >  1:213519/1‑50 (MQ=255)

      aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       >  1:4180828/1‑50 (MQ=255)

      aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       <  1:1714526/50‑1 (MQ=255)

       tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                      <  1:2748458/50‑1 (MQ=255)

       tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                      >  1:4571779/1‑50 (MQ=255)

        cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                      >  1:2728592/1‑49 (MQ=255)

        cGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                      >  1:3244333/1‑49 (MQ=255)

         gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                    <  1:5710134/50‑1 (MQ=255)

         gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                    >  1:8372985/1‑50 (MQ=255)

         gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                    <  1:1966529/50‑1 (MQ=255)

          ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                   <  1:2412491/50‑1 (MQ=255)

           tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                   >  1:8599005/1‑49 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                 >  1:8241331/1‑50 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                 >  1:7596273/1‑50 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                  <  1:2145527/49‑1 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                  <  1:4304497/49‑1 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                  <  1:2314438/49‑1 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                  <  1:3175320/49‑1 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                  <  1:6633192/49‑1 (MQ=255)

            aTGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATa                                  <  1:3522697/49‑1 (MQ=255)

             tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                 <  1:6087105/49‑1 (MQ=255)

             tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                                <  1:7961658/50‑1 (MQ=255)

             tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                                >  1:8003047/1‑50 (MQ=255)

             tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAAc                                <  1:6604006/50‑1 (MQ=255)

              gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                               >  1:8123684/1‑50 (MQ=255)

               ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                               >  1:5083551/1‑49 (MQ=255)

               ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                               <  1:7304108/49‑1 (MQ=255)

                cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCa                             <  1:4975068/50‑1 (MQ=255)

                 gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                            >  1:7492050/1‑50 (MQ=255)

                 gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                            <  1:1946020/50‑1 (MQ=255)

                 gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                            <  1:4101312/50‑1 (MQ=255)

                 gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                            <  1:7625483/50‑1 (MQ=255)

                  ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                           <  1:5234516/50‑1 (MQ=255)

                  ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                           >  1:6019214/1‑50 (MQ=255)

                  ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                           >  1:2771155/1‑50 (MQ=255)

                    aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                          <  1:4270638/49‑1 (MQ=255)

                     gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                         <  1:3173491/49‑1 (MQ=255)

                     gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                          >  1:1005384/1‑48 (MQ=255)

                        ttCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      >  1:4242227/1‑49 (MQ=255)

                        ttCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      >  1:8107063/1‑49 (MQ=255)

                        ttCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      >  1:229200/1‑49 (MQ=255)

                          cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      <  1:3040111/47‑1 (MQ=255)

                          cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      <  1:6080600/47‑1 (MQ=255)

                           gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                  <  1:6115655/50‑1 (MQ=255)

                           gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                   <  1:8397867/49‑1 (MQ=255)

                            ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                  >  1:5284517/1‑49 (MQ=255)

                            ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                  >  1:4491408/1‑49 (MQ=255)

                            ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                 <  1:4719985/50‑1 (MQ=255)

                            ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGc                 <  1:2218825/50‑1 (MQ=255)

                             ctctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa                <  1:7675306/50‑1 (MQ=255)

                              tctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCa                >  1:2768711/1‑49 (MQ=255)

                               ctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               >  1:2471760/1‑49 (MQ=255)

                               ctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               >  1:4403812/1‑49 (MQ=255)

                                tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa             >  1:2260447/1‑50 (MQ=255)

                                tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               <  1:784582/48‑1 (MQ=255)

                                tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               <  1:4540003/48‑1 (MQ=255)

                                tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               <  1:7777256/48‑1 (MQ=255)

                                tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               <  1:2425551/48‑1 (MQ=255)

                                 aTTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            >  1:1438613/1‑50 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa             <  1:4827174/48‑1 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaa              <  1:8475715/47‑1 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaa              <  1:821343/47‑1 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            <  1:1952211/49‑1 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            >  1:2443427/1‑49 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            >  1:6175022/1‑49 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            <  1:1219363/49‑1 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATc           <  1:1072433/50‑1 (MQ=255)

                                  ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATc           <  1:2843541/50‑1 (MQ=255)

                                   tCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            >  1:6086024/1‑48 (MQ=255)

                                    cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCa          <  1:3183694/49‑1 (MQ=255)

                                    cTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCa          <  1:3546275/49‑1 (MQ=255)

                                     tGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt        >  1:3453845/1‑50 (MQ=255)

                                     tGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt        >  1:4996226/1‑50 (MQ=255)

                                      gAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt       >  1:5894333/1‑50 (MQ=255)

                                       aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAg         <  1:3967655/47‑1 (MQ=255)

                                       aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGttt      >  1:3526797/1‑50 (MQ=255)

                                       aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt       <  1:3341678/49‑1 (MQ=255)

                                       aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtt       <  1:3763378/49‑1 (MQ=255)

                                       aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt        <  1:457676/48‑1 (MQ=255)

                                        gcgcGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt     <  1:7957523/50‑1 (MQ=255)

                                        gcgcGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt     <  1:5478245/50‑1 (MQ=255)

                                         cgcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTc    >  1:1352484/1‑50 (MQ=255)

                                          gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt   >  1:6216139/1‑50 (MQ=255)

                                          gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt   <  1:1336644/50‑1 (MQ=255)

                                          gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt   <  1:4539056/50‑1 (MQ=255)

                                           cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt   >  1:2540516/1‑49 (MQ=255)

                                           cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt   >  1:4120784/1‑49 (MQ=255)

                                           cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTg  <  1:3937661/50‑1 (MQ=255)

                                           cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTg  <  1:5643166/50‑1 (MQ=255)

                                           cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTg  <  1:4754193/50‑1 (MQ=255)

CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTG  >  NC_000913/1811162‑1811254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`