BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	405.2 / NA	102	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (45/57); total (45/57)

Only 100 of 105 total aligned reads displayed.

GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214839‑214931

gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                              <  1:2512902/49‑1 (MQ=255)

 tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             <  1:6214833/49‑1 (MQ=255)

 tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:4406668/1‑49 (MQ=255)

 tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:648052/1‑49 (MQ=255)

   aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          <  1:3980025/50‑1 (MQ=255)

   aTTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAt                                          <  1:776616/50‑1 (MQ=255)

    tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                         <  1:2905605/50‑1 (MQ=255)

      tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        <  1:1786291/49‑1 (MQ=255)

      tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       >  1:3967938/1‑50 (MQ=255)

       cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCg                                       <  1:3436176/49‑1 (MQ=255)

       cccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                      >  1:6788117/1‑50 (MQ=255)

        ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                      <  1:1368181/49‑1 (MQ=255)

        ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:4430565/50‑1 (MQ=255)

        ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     >  1:4572324/1‑50 (MQ=255)

        ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     >  1:4773693/1‑50 (MQ=255)

        ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:3133536/50‑1 (MQ=255)

         cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  1:8552402/50‑1 (MQ=255)

         cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    >  1:4206238/1‑50 (MQ=255)

         cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    >  1:7233420/1‑50 (MQ=255)

         cGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgc                                    <  1:7968278/50‑1 (MQ=255)

          gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   <  1:8105251/50‑1 (MQ=255)

          gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:4686807/1‑50 (MQ=255)

          gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:3361797/1‑50 (MQ=255)

           aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:5441995/1‑49 (MQ=255)

            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:5608118/1‑48 (MQ=255)

            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:7223464/1‑48 (MQ=255)

            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:1917494/1‑48 (MQ=255)

            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCa                                 <  1:190138/50‑1 (MQ=255)

            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCa                                 <  1:3210435/50‑1 (MQ=255)

            cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCa                                 <  1:2576132/50‑1 (MQ=255)

             aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                <  1:6843593/50‑1 (MQ=255)

             aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                <  1:7809041/50‑1 (MQ=255)

             aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                <  1:3632510/50‑1 (MQ=255)

             aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                >  1:4625205/1‑50 (MQ=255)

             aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGAAg                                <  1:4822424/50‑1 (MQ=38)

              tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:7848209/1‑50 (MQ=255)

              tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:4349088/1‑50 (MQ=255)

              tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  1:2585934/50‑1 (MQ=255)

                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             >  1:3880522/1‑50 (MQ=255)

                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             >  1:7424844/1‑50 (MQ=255)

                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             <  1:3588051/50‑1 (MQ=255)

                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             <  1:2347401/50‑1 (MQ=255)

                ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                             <  1:578804/50‑1 (MQ=255)

                  gCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           >  1:887744/1‑50 (MQ=255)

                   cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTGGTGCGCAGGGcgcg                          >  1:7190554/1‑50 (MQ=255)

                   cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgcgc                           <  1:1190993/49‑1 (MQ=255)

                   cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          >  1:2859708/1‑50 (MQ=255)

                   cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          >  1:5231617/1‑50 (MQ=255)

                    tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  1:5827647/1‑50 (MQ=255)

                    tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  1:1230529/1‑50 (MQ=255)

                    tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                         >  1:3616302/1‑50 (MQ=255)

                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       <  1:1489487/50‑1 (MQ=255)

                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       <  1:6275015/50‑1 (MQ=255)

                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       <  1:8428173/50‑1 (MQ=255)

                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                       >  1:7094800/1‑50 (MQ=255)

                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        <  1:7520344/49‑1 (MQ=255)

                      ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        <  1:3680143/49‑1 (MQ=255)

                        cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     >  1:4432657/1‑50 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     <  1:3126009/49‑1 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:5001817/1‑50 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    <  1:8007782/50‑1 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    <  1:6873303/50‑1 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:7229006/1‑50 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:7962509/1‑50 (MQ=255)

                         ccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGAGCgcgggcgg                     <  1:3944715/49‑1 (MQ=38)

                          cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc                   <  1:5683659/50‑1 (MQ=255)

                             tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagc                <  1:4388692/50‑1 (MQ=255)

                             tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  1:44716/1‑49 (MQ=255)

                               gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca               <  1:6941785/49‑1 (MQ=255)

                               gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCAt              >  1:1960295/1‑50 (MQ=255)

                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             >  1:7644167/1‑50 (MQ=255)

                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:736454/50‑1 (MQ=255)

                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             >  1:502364/1‑50 (MQ=255)

                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:2162225/50‑1 (MQ=255)

                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:3530455/50‑1 (MQ=255)

                                tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:1153026/50‑1 (MQ=255)

                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:6272326/1‑50 (MQ=255)

                                 gAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:4453723/1‑50 (MQ=255)

                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           <  1:1282389/50‑1 (MQ=255)

                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:228361/1‑49 (MQ=255)

                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  1:4567065/49‑1 (MQ=255)

                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            >  1:7270699/1‑49 (MQ=255)

                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  1:5453067/49‑1 (MQ=255)

                                  aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt            <  1:3243180/49‑1 (MQ=255)

                                   gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:3504149/50‑1 (MQ=255)

                                   gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:2479720/50‑1 (MQ=255)

                                    cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:690841/1‑50 (MQ=255)

                                    cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:8238148/1‑50 (MQ=255)

                                     ttGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa         >  1:4383205/1‑49 (MQ=255)

                                      tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  1:7321373/1‑49 (MQ=255)

                                      tGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        >  1:5301322/1‑49 (MQ=255)

                                       gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  1:3280361/49‑1 (MQ=255)

                                       gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        <  1:2976259/48‑1 (MQ=255)

                                       gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat        <  1:462329/48‑1 (MQ=255)

                                        agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaataa      <  1:8294150/49‑1 (MQ=255)

                                        agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg     <  1:1887286/50‑1 (MQ=255)

                                          atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   >  1:1979588/1‑50 (MQ=255)

                                          atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   <  1:7333252/50‑1 (MQ=255)

                                          atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   <  1:5687489/50‑1 (MQ=255)

                                           tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  <  1:2334133/50‑1 (MQ=255)

GTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214839‑214931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`