| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,316,813 | G→A | A213T (GCA→ACA) | rcsB → | DNA‑binding transcriptional activator RcsB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,316,813 | 0 | G | A | 88.0% | 370.2 / 34.1 | 117 | A213T (GCA→ACA) | rcsB | DNA‑binding transcriptional activator RcsB |
| Reads supporting (aligned to +/- strand): ref base G (5/9); new base A (45/58); total (50/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTT > NC_000913/2316764‑2316862 | aCGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACa < 1:4151890/50‑1 (MQ=255) cGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAg < 1:7542569/50‑1 (MQ=255) cGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAg < 1:3261603/50‑1 (MQ=255) gATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg > 1:3872923/1‑46 (MQ=255) atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGa > 1:6866716/1‑49 (MQ=255) atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGa > 1:832651/1‑49 (MQ=255) atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGAt < 1:1060423/50‑1 (MQ=255) atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGa > 1:2858521/1‑49 (MQ=255) atatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGAt < 1:4210388/50‑1 (MQ=255) tatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATa < 1:2209150/50‑1 (MQ=255) tatCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATa < 1:798685/50‑1 (MQ=255) tCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATa > 1:4711651/1‑48 (MQ=255) gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAg < 1:1569839/49‑1 (MQ=255) gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAg < 1:3646058/49‑1 (MQ=255) gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGa < 1:4955200/50‑1 (MQ=255) gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGa > 1:4467241/1‑50 (MQ=255) gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGa < 1:3147226/50‑1 (MQ=255) gCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGa > 1:2952686/1‑50 (MQ=255) cccTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGAc > 1:53551/1‑50 (MQ=255) ccTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGAc > 1:4550154/1‑49 (MQ=255) ccTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGAc > 1:2416036/1‑49 (MQ=255) ccTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACt < 1:13283/50‑1 (MQ=255) ccTGCTGAATTATCGCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACt < 1:3705475/50‑1 (MQ=255) gctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAAt > 1:7507394/1‑50 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAAt < 1:1413616/49‑1 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTaa < 1:7042407/48‑1 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTaa < 1:3996298/48‑1 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAAt < 1:4296527/49‑1 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAAt < 1:775191/49‑1 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAAt > 1:5653699/1‑49 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATc < 1:1941971/50‑1 (MQ=255) ctgAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATc < 1:7572630/50‑1 (MQ=255) gAATTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCAc > 1:5486119/1‑50 (MQ=255) aaTTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCa < 1:5672655/48‑1 (MQ=255) aaTTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCAcc > 1:3212182/1‑50 (MQ=255) aaTTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCAc < 1:5192779/49‑1 (MQ=255) aaTTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCAc < 1:3025031/49‑1 (MQ=255) aTTATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCt < 1:8495962/50‑1 (MQ=255) ttATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTg < 1:6739846/50‑1 (MQ=255) ttATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTg < 1:5491735/50‑1 (MQ=255) ttATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTg < 1:4871033/50‑1 (MQ=255) tATCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTg > 1:1105211/1‑49 (MQ=255) aTCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTg > 1:3434996/1‑48 (MQ=255) aTCTCTCTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGt < 1:3348897/49‑1 (MQ=255) tctctcTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTa < 1:5329227/49‑1 (MQ=255) tctctcTTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTa < 1:6567060/49‑1 (MQ=255) tctctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:1684353/1‑50 (MQ=255) tctctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:8320137/1‑50 (MQ=255) ctctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:455154/1‑49 (MQ=255) tctTCAGTGACCTTAAGTCCTACAGATAAAGACTAATCACCTGTAg < 1:2097727/46‑1 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAgg < 1:6190568/47‑1 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAg < 1:890585/46‑1 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAg < 1:1071496/46‑1 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:4320694/1‑48 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc < 1:622577/48‑1 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:5530264/1‑48 (MQ=255) tctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc < 1:3960226/48‑1 (MQ=255) tctTCAGTGACCTTAAGACCGACAGATAAAGACTAATCACCTGTAGGc > 1:8254625/1‑48 (MQ=255) ctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:428801/1‑47 (MQ=255) ctTCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGc > 1:5055571/1‑47 (MQ=255) ttCAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGcc < 1:7956035/47‑1 (MQ=255) cAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGa < 1:7936691/48‑1 (MQ=255) cAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGAt > 1:2731437/1‑49 (MQ=255) cAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAg < 1:1359991/47‑1 (MQ=255) cAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGAt < 1:8532884/49‑1 (MQ=255) cAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGAt < 1:8338005/49‑1 (MQ=255) cAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGAt > 1:2066333/1‑49 (MQ=255) cAGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGAt < 1:7003154/49‑1 (MQ=255) aGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATaa < 1:5018105/50‑1 (MQ=255) aGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGAt > 1:7275893/1‑48 (MQ=255) aGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATaa < 1:420611/50‑1 (MQ=255) aGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATaa < 1:2370922/50‑1 (MQ=255) aGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATaa < 1:6406345/50‑1 (MQ=255) aGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATa < 1:8034194/49‑1 (MQ=255) aGTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATa < 1:3085728/49‑1 (MQ=255) gTGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGAt > 1:1172773/1‑47 (MQ=255) tGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGa > 1:4785839/1‑50 (MQ=255) tGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGa < 1:4012765/50‑1 (MQ=255) tGACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGa > 1:5400056/1‑50 (MQ=255) gACCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGAc > 1:4120050/1‑50 (MQ=255) aCCTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGAcg > 1:5203466/1‑50 (MQ=255) ccTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGAcgc > 1:4900655/1‑50 (MQ=255) ccTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGAcgc < 1:2292847/50‑1 (MQ=255) ccTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGAcgc < 1:326946/50‑1 (MQ=255) cTTAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGAcgcg < 1:2469397/50‑1 (MQ=255) tAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGt > 1:27103/1‑49 (MQ=255) tAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGt > 1:3337008/1‑49 (MQ=255) tAAGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGt > 1:5890511/1‑49 (MQ=255) aaGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGtt < 1:79293/49‑1 (MQ=255) aaGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGtt < 1:1218442/49‑1 (MQ=255) aaGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGt > 1:1588313/1‑48 (MQ=255) aGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTa < 1:3217636/49‑1 (MQ=255) aGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTa < 1:2111802/49‑1 (MQ=255) aGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTa < 1:3744137/49‑1 (MQ=255) aGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAg > 1:5669247/1‑50 (MQ=255) aGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAg > 1:8565516/1‑50 (MQ=255) aGTCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAg < 1:2770105/50‑1 (MQ=255) tCCGACAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAgtg < 1:3076667/50‑1 (MQ=255) ggCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCt > 1:83826/1‑50 (MQ=255) gCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCtt < 1:586033/50‑1 (MQ=255) | GAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATAAAGACTAATCACCTGTAGGCCAGATAAGACGCGTTAGTGTCTT > NC_000913/2316764‑2316862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |