| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,662,004 | T→C | E43G (GAA→GGA) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,662,004 | 0 | T | C | 94.4% | 325.0 / 6.8 | 89 | E43G (GAA→GGA) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (40/44); total (44/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
GTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACG > NC_000913/2661957‑2662052 | gTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTccc < 1:2019571/50‑1 (MQ=255) tACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa < 1:2201468/50‑1 (MQ=255) tACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa < 1:1298202/50‑1 (MQ=255) cGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAg < 1:1652818/49‑1 (MQ=255) gCTGGAAACCAGACCATTTTTACGCAGACGGGAGAACAGTTGTCCCAGAt < 1:8137977/50‑1 (MQ=255) gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGa > 1:6213811/1‑49 (MQ=255) gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt < 1:2939656/50‑1 (MQ=255) gCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt < 1:3307912/50‑1 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 1:1491018/1‑49 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 1:5785884/1‑49 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATa < 1:7348412/50‑1 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATa < 1:3959510/50‑1 (MQ=255) cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGCCCCAGAt > 1:6670097/1‑49 (MQ=39) tGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt > 1:1756957/1‑48 (MQ=255) gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGa < 1:4886056/50‑1 (MQ=255) gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa < 1:6193539/48‑1 (MQ=255) gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATaa < 1:8147189/48‑1 (MQ=255) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa < 1:7294770/50‑1 (MQ=255) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa < 1:837742/50‑1 (MQ=255) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaa < 1:8596708/50‑1 (MQ=255) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa > 1:6960793/1‑49 (MQ=255) aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa > 1:3978993/1‑49 (MQ=255) aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa > 1:8253074/1‑48 (MQ=255) aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa > 1:1242938/1‑48 (MQ=255) aCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAg < 1:2592061/50‑1 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg > 1:7981996/1‑50 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg < 1:3278427/50‑1 (MQ=255) ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg < 1:3802526/50‑1 (MQ=255) cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAggg < 1:1244147/50‑1 (MQ=255) cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg > 1:5277175/1‑49 (MQ=255) aGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAggg > 1:5486938/1‑49 (MQ=255) aGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAggg > 1:3825827/1‑49 (MQ=255) aGACCATTTGTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGa < 1:3260966/50‑1 (MQ=255) gACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaa < 1:8278199/50‑1 (MQ=255) gACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGa > 1:5805661/1‑49 (MQ=255) aCCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGaaa > 1:1564525/1‑50 (MQ=255) ccATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAt < 1:3885865/50‑1 (MQ=255) ccATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAt < 1:3379133/50‑1 (MQ=255) ccATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAt > 1:4076846/1‑50 (MQ=255) cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt < 1:6573452/50‑1 (MQ=255) cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt > 1:1704623/1‑50 (MQ=255) cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt > 1:6791613/1‑50 (MQ=255) aTTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTc > 1:507303/1‑50 (MQ=255) tttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTcc > 1:5313101/1‑50 (MQ=255) ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc > 1:1071393/1‑50 (MQ=255) ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc > 1:2879408/1‑50 (MQ=255) tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc < 1:2149442/49‑1 (MQ=255) ttACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCt > 1:2902451/1‑49 (MQ=255) ttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt < 1:4853389/49‑1 (MQ=255) tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTg < 1:5636823/49‑1 (MQ=255) tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa > 1:1403009/1‑50 (MQ=255) aCGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc > 1:1279226/1‑50 (MQ=255) cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc < 1:5840903/49‑1 (MQ=255) cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc < 1:3805556/49‑1 (MQ=255) gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt > 1:7084776/1‑50 (MQ=255) gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt > 1:2587181/1‑50 (MQ=255) gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt > 1:2309269/1‑50 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt > 1:2898161/1‑50 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt > 1:3791735/1‑50 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt < 1:5711759/50‑1 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt < 1:3936827/50‑1 (MQ=255) cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt > 1:5198797/1‑50 (MQ=255) gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg < 1:830777/50‑1 (MQ=255) gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg < 1:7432772/50‑1 (MQ=255) aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg < 1:8167239/50‑1 (MQ=255) aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg > 1:6981235/1‑49 (MQ=255) cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg > 1:6551582/1‑49 (MQ=255) cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa < 1:1303092/50‑1 (MQ=255) gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaa < 1:2038651/50‑1 (MQ=255) gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa > 1:2850172/1‑49 (MQ=255) gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa > 1:6008964/1‑49 (MQ=255) ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa < 1:5935289/50‑1 (MQ=255) ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa > 1:3871700/1‑50 (MQ=255) gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat < 1:3844058/50‑1 (MQ=255) gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat < 1:30647/50‑1 (MQ=255) gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat < 1:545131/50‑1 (MQ=255) aaaaCAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata > 1:1521605/1‑50 (MQ=255) aaaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata > 1:7755525/1‑50 (MQ=255) aaaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata < 1:1521553/50‑1 (MQ=255) aaaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata > 1:1405520/1‑50 (MQ=255) aaaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAata < 1:4227166/50‑1 (MQ=255) aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat < 1:7634865/50‑1 (MQ=255) aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 1:1470269/1‑50 (MQ=255) aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 1:5527252/1‑49 (MQ=255) aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 1:4214716/1‑49 (MQ=255) aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc < 1:6309787/50‑1 (MQ=255) aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat > 1:4274531/1‑48 (MQ=255) aCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCa < 1:3760574/50‑1 (MQ=255) cAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg > 1:5977145/1‑50 (MQ=255) aGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc < 1:3684820/50‑1 (MQ=255) gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc < 1:8038191/50‑1 (MQ=255) gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc < 1:6450789/50‑1 (MQ=255) gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc < 1:1578853/50‑1 (MQ=255) gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc < 1:8462396/50‑1 (MQ=255) ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc > 1:4211533/1‑49 (MQ=255) ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa < 1:6135789/50‑1 (MQ=255) tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa > 1:6790940/1‑50 (MQ=255) tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa > 1:5782451/1‑50 (MQ=255) tgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCaa > 1:5537837/1‑50 (MQ=255) ttCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACg > 1:2391687/1‑50 (MQ=255) | GTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCAACG > NC_000913/2661957‑2662052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |