BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	461.9 / NA	115	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (58/57); total (58/57)

Only 100 of 115 total aligned reads displayed.

CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048

cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:3486553/1‑50 (MQ=255)

 ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:1257681/1‑50 (MQ=255)

  gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          <  1:2462776/50‑1 (MQ=255)

  gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          <  1:3249728/50‑1 (MQ=255)

  gAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          <  1:5632172/50‑1 (MQ=255)

   aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:7339552/50‑1 (MQ=255)

   aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:4440522/50‑1 (MQ=255)

   aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:3425045/1‑49 (MQ=255)

    gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:5290625/1‑48 (MQ=255)

    gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:7209694/50‑1 (MQ=255)

    gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:7005178/50‑1 (MQ=255)

     tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       <  1:5058656/50‑1 (MQ=255)

       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:6874458/49‑1 (MQ=255)

       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagc                                      <  1:4705250/49‑1 (MQ=255)

        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    >  1:1801329/1‑50 (MQ=255)

        gTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagcag                                    <  1:7078713/50‑1 (MQ=255)

         tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:6307466/1‑50 (MQ=255)

         tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:5794771/1‑50 (MQ=255)

          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:559532/1‑50 (MQ=255)

          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:2627405/1‑50 (MQ=255)

           aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 <  1:8362350/50‑1 (MQ=255)

            ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:6566683/1‑49 (MQ=255)

            ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCg                                <  1:2073206/50‑1 (MQ=255)

             cGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               <  1:8618054/50‑1 (MQ=255)

              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:7045428/1‑49 (MQ=255)

              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:7542735/1‑49 (MQ=255)

              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:2580207/1‑49 (MQ=255)

               cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGAt                              >  1:5592786/1‑49 (MQ=255)

               cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:1151727/50‑1 (MQ=255)

               cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:6200035/50‑1 (MQ=255)

                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:7199600/50‑1 (MQ=255)

                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:8414298/50‑1 (MQ=255)

                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            >  1:5727222/1‑50 (MQ=255)

                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:548676/50‑1 (MQ=255)

                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:3776490/50‑1 (MQ=255)

                 cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:2092518/50‑1 (MQ=255)

                 cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           <  1:6144157/50‑1 (MQ=255)

                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:7811277/1‑50 (MQ=255)

                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          <  1:2791796/50‑1 (MQ=255)

                   tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:1765757/1‑50 (MQ=255)

                   tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         <  1:7232019/50‑1 (MQ=255)

                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGtt                        <  1:839565/49‑1 (MQ=255)

                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:691658/1‑50 (MQ=255)

                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:7162839/1‑50 (MQ=255)

                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:7409673/1‑50 (MQ=255)

                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:8048016/1‑50 (MQ=255)

                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:7926097/1‑50 (MQ=255)

                       aTTTTCTTCGCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      <  1:2136683/49‑1 (MQ=39)

                       aTTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAtt                     >  1:1089804/1‑50 (MQ=255)

                         tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:8513485/1‑50 (MQ=255)

                         tttCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:293582/1‑50 (MQ=255)

                          ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   <  1:4624462/49‑1 (MQ=255)

                           tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCg                  <  1:878855/49‑1 (MQ=255)

                            cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  1:2131965/49‑1 (MQ=255)

                            cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 <  1:557145/49‑1 (MQ=255)

                            cttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                >  1:6776265/1‑50 (MQ=255)

                              tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:3803307/1‑50 (MQ=255)

                              tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:3047541/1‑50 (MQ=255)

                              tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:8075688/1‑50 (MQ=255)

                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:4836584/48‑1 (MQ=255)

                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:2531564/48‑1 (MQ=255)

                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAtt               <  1:5505854/48‑1 (MQ=255)

                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                <  1:7579298/47‑1 (MQ=255)

                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              <  1:1900497/49‑1 (MQ=255)

                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:391110/1‑50 (MQ=255)

                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:5376944/50‑1 (MQ=255)

                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:5826557/50‑1 (MQ=255)

                                aCTACCGATTTCGGGTCTTCCAGCACGCCGATAACGTTATTGCGATTATc            >  1:2683880/1‑50 (MQ=38)

                                aCTACCGATTTCGGGTCTACCAACAGGCCGATAACGTTATTGCGATTATc            <  1:1516965/50‑1 (MQ=38)

                                 cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           >  1:1821303/1‑50 (MQ=255)

                                 cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           >  1:5091746/1‑50 (MQ=255)

                                  tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2057134/1‑50 (MQ=255)

                                  tACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:4315473/1‑50 (MQ=255)

                                   aCCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:4279127/1‑49 (MQ=255)

                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:3114019/1‑48 (MQ=255)

                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:2189955/1‑48 (MQ=255)

                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:5648964/48‑1 (MQ=255)

                                    ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCa        <  1:6067839/50‑1 (MQ=255)

                                     cGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          >  1:1705283/1‑47 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:3661785/50‑1 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:1545109/1‑50 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:2865405/50‑1 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:6490430/50‑1 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:2655875/1‑50 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:1886970/50‑1 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:2289380/50‑1 (MQ=255)

                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:7794681/1‑50 (MQ=255)

                                       aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:5217259/1‑50 (MQ=255)

                                       aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:486322/1‑50 (MQ=255)

                                       aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:6181865/1‑50 (MQ=255)

                                        tttCGGGTCTTCCAGCAGGTCGATAACGTTATTGCGATTATCGTCAGACt    >  1:3841895/1‑50 (MQ=39)

                                        tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    <  1:7569334/50‑1 (MQ=255)

                                        tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    <  1:4042395/50‑1 (MQ=255)

                                        tttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACt    <  1:1035870/50‑1 (MQ=255)

                                         ttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   >  1:2013319/1‑50 (MQ=255)

                                         ttCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   >  1:3889747/1‑50 (MQ=255)

                                          tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:1019390/49‑1 (MQ=255)

                                          tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACtt   <  1:2312383/49‑1 (MQ=255)

                                          tCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:3159018/1‑50 (MQ=255)

                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:4674427/1‑49 (MQ=255)

CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`